BACKGROUND/AIMS: Mannose-binding lectin, a key factor of the innate immune system, has genetic polymorphism, and individuals who carry certain genotypes of mannose-binding lectin are known to be more prone to severe or prolonged infectious diseases. We aimed to find any relevance of mannose-binding lectin polymorphism to hepatitis C virus infection. METHODS: We determined the mannose-binding lectin genotypes by sequence specific priming-polymerase chain reaction in 159 hepatitis C virus-infected chronic hepatitis patients and 218 healthy controls in Japan by looking at 4 polymorphic loci: 2 (H/L and X/Y) within the promoter region and 2 (P/Q and A/B) within exon-1 of the mannose-binding lectin gene. RESULTS: A group of mannose-binding lectin genotypes designated "XB-type" (containing LXPA or LYPB haplotype at least heterozygously) was less frequently found in interferon-responsive patients (38.5%) than in interferon-resistant patients (60.7%, p=0.008) and controls (57.3%, p=0.014). Individuals with the "XB-type" had lower serum concentrations of mannose-binding lectin, compared to those with "YA-type", which is defined by homozygous carriage of both Y and A alleles: 0.63+/-0.61 vs 2.06+/-1.17 mg/l, p<0.001. CONCLUSIONS: Our results suggest that the mannose-binding lectin-related innate immune system plays an important role in elimination of hepatitis C virus during interferon therapy. Determining mannose-binding lectin genotypes may help in selecting the hepatitis C virus-infected patients to be treated with interferon.
BACKGROUND/AIMS: Mannose-binding lectin, a key factor of the innate immune system, has genetic polymorphism, and individuals who carry certain genotypes of mannose-binding lectin are known to be more prone to severe or prolonged infectious diseases. We aimed to find any relevance of mannose-binding lectin polymorphism to hepatitis C virus infection. METHODS: We determined the mannose-binding lectin genotypes by sequence specific priming-polymerase chain reaction in 159 hepatitis C virus-infected chronic hepatitispatients and 218 healthy controls in Japan by looking at 4 polymorphic loci: 2 (H/L and X/Y) within the promoter region and 2 (P/Q and A/B) within exon-1 of the mannose-binding lectin gene. RESULTS: A group of mannose-binding lectin genotypes designated "XB-type" (containing LXPA or LYPB haplotype at least heterozygously) was less frequently found in interferon-responsive patients (38.5%) than in interferon-resistant patients (60.7%, p=0.008) and controls (57.3%, p=0.014). Individuals with the "XB-type" had lower serum concentrations of mannose-binding lectin, compared to those with "YA-type", which is defined by homozygous carriage of both Y and A alleles: 0.63+/-0.61 vs 2.06+/-1.17 mg/l, p<0.001. CONCLUSIONS: Our results suggest that the mannose-binding lectin-related innate immune system plays an important role in elimination of hepatitis C virus during interferon therapy. Determining mannose-binding lectin genotypes may help in selecting the hepatitis C virus-infectedpatients to be treated with interferon.
Authors: Melinda M Dean; Robert L Flower; Damon P Eisen; Robyn M Minchinton; Derek N J Hart; Slavica Vuckovic Journal: Immunology Date: 2010-11-23 Impact factor: 7.397
Authors: K S Brown; M J Keogh; N Tagiuri; M J Grainge; J S Presanis; S D Ryder; W L Irving; J K Ball; R B Sim; T P Hickling Journal: Clin Exp Immunol Date: 2007-01 Impact factor: 4.330
Authors: M L Alves Pedroso; A B W Boldt; L Pereira-Ferrari; R Steffensen; E Strauss; J C Jensenius; S O Ioshii; I Messias-Reason Journal: Clin Exp Immunol Date: 2008-03-10 Impact factor: 4.330
Authors: Kristelle S Brown; Michael J Keogh; Ania M Owsianka; Richard Adair; Arvind H Patel; James N Arnold; Jonathan K Ball; Robert B Sim; Alexander W Tarr; Timothy P Hickling Journal: Protein Cell Date: 2010-07-29 Impact factor: 14.870