K Stelwagen1, D J Ormrod. 1. Dairy Sciences, AgResearch, Ruakura Agricultural Centre, Hamilton, New Zealand. stelwagenk@agresearch.cri.nz
Abstract
OBJECTIVE AND DESIGN: Effects of hyperimmune milk factor (HIMF), an anti-inflammatory factor from milk of hyperimmunised cows, on tight junction permeability and cell growth were studied in vitro. MATERIAL OR SUBJECTS: Mammary (HC11) and kidney (MDCK) epithelial cell lines were used. TREATMENT: HIMF was used at a final concentration of 2 mg/ml. METHODS: Tight junction permeability was assessed by measuring transepithelial electrical resistance (TER) across confluent monolayers, following the addition of HIMF with or without an inflammatory challenge. Cell growth was assessed by measuring total DNA of cultures with and without HIMF. Data were analysed by analyses of variance. RESULTS: HIMF promoted tight junction formation and prevented loss of TER following a challenge in both epithelia. Post-challenge recovery of TER was also faster with HIMF. HIMF inhibited cell growth. CONCLUSIONS: HIMF stimulates tight junction maintenance and formation, and its previously reported anti-inflammatory properties may be mediated by restricting the extravasation of white blood cells through tight junctions.
OBJECTIVE AND DESIGN: Effects of hyperimmune milk factor (HIMF), an anti-inflammatory factor from milk of hyperimmunised cows, on tight junction permeability and cell growth were studied in vitro. MATERIAL OR SUBJECTS: Mammary (HC11) and kidney (MDCK) epithelial cell lines were used. TREATMENT: HIMF was used at a final concentration of 2 mg/ml. METHODS: Tight junction permeability was assessed by measuring transepithelial electrical resistance (TER) across confluent monolayers, following the addition of HIMF with or without an inflammatory challenge. Cell growth was assessed by measuring total DNA of cultures with and without HIMF. Data were analysed by analyses of variance. RESULTS:HIMF promoted tight junction formation and prevented loss of TER following a challenge in both epithelia. Post-challenge recovery of TER was also faster with HIMF. HIMF inhibited cell growth. CONCLUSIONS:HIMF stimulates tight junction maintenance and formation, and its previously reported anti-inflammatory properties may be mediated by restricting the extravasation of white blood cells through tight junctions.