Literature DB >> 982538

Plateletpheresis residues: a source of large quantities of human blood lymphocytes.

G B Segel, M A Lichtman, B R Gordon, J L MacPherson, J Nusbacher.   

Abstract

The residue from single-donor plateletpheresis contains a large number of human mononuclear cells. We have been able to harvest more than 1 X 10(9) viable lymphocytes for laboratory study from the leukocyte-rich sediment that previously had been discarded. Prior to removal by adherence 5 X 10(8) monocytes were also available for future purification, if desired. The physical properties and response to phytohemagglutinin were very similar when lymphocytes isolated from plateletpheresis residues were compared with those obtained directly from veneous blood.

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Year:  1976        PMID: 982538     DOI: 10.1046/j.1537-2995.1976.16577039302.x

Source DB:  PubMed          Journal:  Transfusion        ISSN: 0041-1132            Impact factor:   3.157


  10 in total

1.  Regulation of sodium and potassium transport in phytohemagglutinin-stimulated human blood lymphocytes.

Authors:  G B Segel; W Simon; M A Lichtman
Journal:  J Clin Invest       Date:  1979-09       Impact factor: 14.808

2.  Na fluxes in human mononuclear leucocytes.

Authors:  M Brossard; G Dagher
Journal:  Experientia       Date:  1986-12-01

3.  Influence of continuous-flow blood separator leucophoresis on in vitro functions of human lymphocytes.

Authors:  H C Siewertsen; H H Peter; J R Kalden; J Wysk; G Eckert; S Oehl; U W Schäfer; H Deicher
Journal:  Clin Exp Immunol       Date:  1978-06       Impact factor: 4.330

4.  A method for following human lymphocyte traffic using indium-111 oxine labelling.

Authors:  J Wagstaff; C Gibson; N Thatcher; W L Ford; H Sharma; W Benson; D Crowther
Journal:  Clin Exp Immunol       Date:  1981-03       Impact factor: 4.330

5.  Calcium exchange and ionized cytoplasmic calcium in resting and activated human monocytes.

Authors:  S P Scully; G B Segel; M A Lichtman
Journal:  J Clin Invest       Date:  1984-08       Impact factor: 14.808

6.  Multicomponent analysis of amino acid transport in human lymphocytes. Diminished L-system transport in chronic leukemic B lymphocytes.

Authors:  G B Segel; W Simon; M A Lichtman
Journal:  J Clin Invest       Date:  1984-07       Impact factor: 14.808

7.  Potasssium transport in human blood lymphocytes treated with phytohemagglutinin.

Authors:  G B Segel; M A Lichtman
Journal:  J Clin Invest       Date:  1976-12       Impact factor: 14.808

8.  Hyperthermia and human leukocyte functions: effects on response of lymphocytes to mitogen and antigen and bactericidal capacity of monocytes and neutrophils.

Authors:  N J Roberts; R T Steigbigel
Journal:  Infect Immun       Date:  1977-12       Impact factor: 3.441

9.  Phorbol ester restores L-system amino acid transport of B lymphocytes in chronic lymphocytic leukemia.

Authors:  T J Woodlock; G B Segel; M A Lichtman
Journal:  J Clin Invest       Date:  1988-01       Impact factor: 14.808

10.  Fc receptor-mediated binding and endocytosis by human mononuclear phagocytes: monomeric IgG is not endocytosed by U937 cells and monocytes.

Authors:  D H Jones; J Nusbacher; C L Anderson
Journal:  J Cell Biol       Date:  1985-02       Impact factor: 10.539

  10 in total

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