Literature DB >> 9821278

Advances in refolding of proteins produced in E. coli.

H Lilie1, E Schwarz, R Rudolph.   

Abstract

Inclusion body production is a common theme in recombinant protein technology. Hence, renaturation of these inclusion body proteins is a field of increasing interest for gaining large amounts of proteins. Recent developments of renaturation procedures include the inhibition of aggregation during refolding by the application of low molecular weight additives and matrix-bound renaturation techniques.

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Year:  1998        PMID： 9821278     DOI： 10.1016/s0958-1669(98)80035-9

Source DB:  PubMed          Journal:  Curr Opin Biotechnol        ISSN： 0958-1669            Impact factor:   9.740


  63 in total

1.  Proteomics of Mycoplasma genitalium: identification and characterization of unannotated and atypical proteins in a small model genome.

Authors:  S Balasubramanian; T Schneider; M Gerstein; L Regan
Journal:  Nucleic Acids Res       Date:  2000-08-15       Impact factor: 16.971

2.  Characterization of the CRISPR/Cas subtype I-A system of the hyperthermophilic crenarchaeon Thermoproteus tenax.

Authors:  André Plagens; Britta Tjaden; Anna Hagemann; Lennart Randau; Reinhard Hensel
Journal:  J Bacteriol       Date:  2012-03-09       Impact factor: 3.490

3.  The likelihood of aggregation during protein renaturation can be assessed using the second virial coefficient.

Authors:  Jason G S Ho; Anton P J Middelberg; Paul Ramage; Hans P Kocher
Journal:  Protein Sci       Date:  2003-04       Impact factor: 6.725

4.  Hexa-histidin tag position influences disulfide structure but not binding behavior of in vitro folded N-terminal domain of rat corticotropin-releasing factor receptor type 2a.

Authors:  Jana Klose; Norbert Wendt; Sybille Kubald; Eberhard Krause; Klaus Fechner; Michael Beyermann; Michael Bienert; Rainer Rudolph; Sven Rothemund
Journal:  Protein Sci       Date:  2004-08-04       Impact factor: 6.725

5.  Rational design of solution additives for the prevention of protein aggregation.

Authors:  Brian M Baynes; Bernhardt L Trout
Journal:  Biophys J       Date:  2004-09       Impact factor: 4.033

6.  Construction, expression and refolding of a bifunctional fusion protein consisting of C-terminal 12-residue of hirudin-PA and reteplase.

Authors:  Ling Gao; Chun Zhang; Lingling Li; Lan Liang; Xuan Deng; Wutong Wu; Zhiguo Su; Rong Yu
Journal:  Protein J       Date:  2012-04       Impact factor: 2.371

7.  An expression and purification system for the biosynthesis of adenosine receptor peptides for biophysical and structural characterization.

Authors:  Zachary T Britton; Elizabeth I Hanle; Anne S Robinson
Journal:  Protein Expr Purif       Date:  2012-06-19       Impact factor: 1.650

8.  Aggregates of α-chymotrypsinogen anneal to access more stable states.

Authors:  Ronald W Maurer; Alan K Hunter; Anne S Robinson; Christopher J Roberts
Journal:  Biotechnol Bioeng       Date:  2013-11-18       Impact factor: 4.530

9.  Bacterial expression and membrane targeting of the rat complement regulator Crry: a new model anticomplement therapeutic.

Authors:  Deborah A Fraser; Claire L Harris; Richard A G Smith; B Paul Morgan
Journal:  Protein Sci       Date:  2002-10       Impact factor: 6.725

10.  Efficient co-expression of a recombinant staphopain A and its inhibitor staphostatin A in Escherichia coli.

Authors:  Benedykt Wladyka; Katarzyna Puzia; Adam Dubin
Journal:  Biochem J       Date:  2005-01-01       Impact factor: 3.857
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