| Literature DB >> 9820599 |
A M Uglialoro1, D Turbay, P A Pesavento, J C Delgado, F E McKenzie, J G Gribben, D Hartl, E J Yunis, A E Goldfeld.
Abstract
We have identified three new human tumor necrosis factor-alpha (TNF-alpha) promoter polymorphisms with single nucleotide (nt) substitutions at -862, -856, and -574 nt relative to the TNF-alpha transcription start site. The -862 and -856 nt TNF-alpha promoter polymorphisms occur with high frequency in Caucasian and Cambodian individuals and are each non-randomly associated with three extended HLA haplotypes. This study, in which 61 independent TNF-alpha promoters were analyzed spanning from -977 to +93 nt relative to the TNF-alpha mRNA cap site, establishes a new canonical TNF-alpha promoter sequence. Furthermore, we show that none of the three novel polymorphisms at -862, -856 and -574 nt or polymorphisms previously described at positions -238, -308 and +70 have an effect upon TNF-alpha gene expression in activated lymphocytes. Thus, these TNF-alpha promoter polymorphisms likely serve as markers for neighboring genes encoding HLA or other undefined molecules in the MHC that may influence disease susceptibility.Entities:
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Year: 1998 PMID： 9820599 PMCID： PMC2518982 DOI： 10.1111/j.1399-0039.1998.tb03056.x
Source DB: PubMed Journal: Tissue Antigens ISSN： 0001-2815