OBJECTIVES: To test the hypothesis that intravascular light could be delivered via a balloon catheter for arterial photodynamic therapy (PDT). DESIGN: Pig non-injury model. MATERIALS: Clinical catheter equipment. METHODS: Large White pigs (15-20 micrograms) were photosensitised with 5-aminolaevulinic acid (5-ALA) induced protoporphyrin IX (PpIX) at a concentration of 120 mg/kg. Arterial biopsies were taken at intervals between 30 mins and 24 h and frozen sections analysed using a CCD camera to give a temporal profile of fluorescence in each arterial layer. PDT was given to normal arterial segments via a 4 mm transparent PTA balloon inflated so as to occlude flow, but not distend the artery. Animals were culled at 3 and 14 days and the above segments harvested. RESULTS: Fluorescence peaked in the adventitia, intima and medial layers at 1.5, 4 and 6 h respectively. PDT at all time points produced VSMC depletion compared with controls. The degree of depletion mirrored the fluorescence profile of PpIX. CONCLUSIONS: PDT can be delivered via a standard PTA balloon with a transparent channel. This depletes the VSMC population within the arterial wall without complications. Intra-arterial PDT is therefore a potential therapy to reduce the incidence of restenosis post-angioplasty.
OBJECTIVES: To test the hypothesis that intravascular light could be delivered via a balloon catheter for arterial photodynamic therapy (PDT). DESIGN:Pig non-injury model. MATERIALS: Clinical catheter equipment. METHODS:Large Whitepigs (15-20 micrograms) were photosensitised with 5-aminolaevulinic acid (5-ALA) induced protoporphyrin IX (PpIX) at a concentration of 120 mg/kg. Arterial biopsies were taken at intervals between 30 mins and 24 h and frozen sections analysed using a CCD camera to give a temporal profile of fluorescence in each arterial layer. PDT was given to normal arterial segments via a 4 mm transparent PTA balloon inflated so as to occlude flow, but not distend the artery. Animals were culled at 3 and 14 days and the above segments harvested. RESULTS: Fluorescence peaked in the adventitia, intima and medial layers at 1.5, 4 and 6 h respectively. PDT at all time points produced VSMC depletion compared with controls. The degree of depletion mirrored the fluorescence profile of PpIX. CONCLUSIONS: PDT can be delivered via a standard PTA balloon with a transparent channel. This depletes the VSMC population within the arterial wall without complications. Intra-arterial PDT is therefore a potential therapy to reduce the incidence of restenosis post-angioplasty.
Authors: R Waksman; I M Leitch; J Roessler; H Yazdi; R Seabron; F Tio; R W Scott; R I Grove; S Rychnovsky; B Robinson; R Pakala; E Cheneau Journal: Heart Date: 2006-01-06 Impact factor: 5.994
Authors: Volker Eichhorn; Alexander Maerz; Georg Salomon; Irmgard F Blanc; Daniel A Reuter; Alwin E Goetz Journal: World J Urol Date: 2011-11-26 Impact factor: 4.226
Authors: Mariela A Céspedes; Daniel A Saénz; Gustavo H Calvo; Marina González; Alexander J MacRobert; Sinan Battah; Adriana G Casas; Gabriela M Di Venosa Journal: Photochem Photobiol Sci Date: 2021-04-06 Impact factor: 3.982