Literature DB >> 9817898

Rapid PCR detection of Helicobacter pylori-associated virulence and resistance genes directly from gastric biopsy material.

B Björkholm1, R Befrits, B Jaup, L Engstrand.   

Abstract

We have developed a PCR-based method to detect macrolide resistance and the virulence gene cagA in Helicobacter pylori within 24 h, thereby improving the lengthy process of culture-based approaches. Total DNA was prepared directly from stomach biopsy specimens. The procedure proved to be rapid and reliable and could be utilized for diagnostic purposes.

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Year:  1998        PMID： 9817898      PMCID： PMC105265     

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  19 in total

1.  Direct polymerase chain reaction test for detection of Helicobacter pylori in humans and animals.

Authors:  S A Ho; J A Hoyle; F A Lewis; A D Secker; D Cross; N P Mapstone; M F Dixon; J I Wyatt; D S Tompkins; G R Taylor
Journal:  J Clin Microbiol       Date:  1991-11       Impact factor: 5.948

2.  A PCR-oligonucleotide ligation assay to determine the prevalence of 23S rRNA gene mutations in clarithromycin-resistant Helicobacter pylori.

Authors:  G G Stone; D Shortridge; J Versalovic; J Beyer; R K Flamm; D Y Graham; A T Ghoneim; S K Tanaka
Journal:  Antimicrob Agents Chemother       Date:  1997-03       Impact factor: 5.191

Review 3.  Helicobacter pylori--can the mechanisms of pathogenesis guide us towards novel strategies for treatment and prevention?

Authors:  P Falk
Journal:  J Intern Med       Date:  1996-12       Impact factor: 8.989

4.  Cytotoxin production by Campylobacter pylori strains isolated from patients with peptic ulcers and from patients with chronic gastritis only.

Authors:  N Figura; P Guglielmetti; A Rossolini; A Barberi; G Cusi; R A Musmanno; M Russi; S Quaranta
Journal:  J Clin Microbiol       Date:  1989-01       Impact factor: 5.948

5.  Development of a rapid PCR method for identification of Helicobacter pylori in dental plaque and gastric biopsy specimens.

Authors:  J Wahlfors; J H Meurman; J Toskala; A Korhonen; P Alakuijala; E Janatuinen; U M Kärkkkäinen; P Nuutinen; J Jänne
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1995-09       Impact factor: 3.267

6.  Detection of Helicobacter pylori in gastric biopsy tissue by polymerase chain reaction.

Authors:  J T Wang; J T Lin; J C Sheu; J C Yang; D S Chen; T H Wang
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1993-05       Impact factor: 3.267

7.  Mutations in 23S rRNA are associated with clarithromycin resistance in Helicobacter pylori.

Authors:  J Versalovic; D Shortridge; K Kibler; M V Griffy; J Beyer; R K Flamm; S K Tanaka; D Y Graham; M F Go
Journal:  Antimicrob Agents Chemother       Date:  1996-02       Impact factor: 5.191

8.  Rapid detection of Helicobacter pylori in gastric biopsy material by polymerase chain reaction.

Authors:  M Hammar; T Tyszkiewicz; T Wadström; P W O'Toole
Journal:  J Clin Microbiol       Date:  1992-01       Impact factor: 5.948

9.  Cloning and expression of a high-molecular-mass major antigen of Helicobacter pylori: evidence of linkage to cytotoxin production.

Authors:  M K Tummuru; T L Cover; M J Blaser
Journal:  Infect Immun       Date:  1993-05       Impact factor: 3.441

10.  One stomach--one strain: does Helicobacter pylori strain variation influence disease outcome?

Authors:  H Enroth; O Nyrén; L Engstrand
Journal:  Dig Dis Sci       Date:  1999-01       Impact factor: 3.199
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  6 in total

1.  Clarithromycin resistance in Helicobacter pylori and its clinical relevance.

Authors:  Hua-Xiang Xia; Xue-Gong Fan; Nicholas J Talley
Journal:  World J Gastroenterol       Date:  1999-06       Impact factor: 5.742

Review 2.  Molecular detection of antimicrobial resistance.

Authors:  A C Fluit; M R Visser; F J Schmitz
Journal:  Clin Microbiol Rev       Date:  2001-10       Impact factor: 26.132

3.  Direct detection of Helicobacter pylori mutations associated with macrolide resistance in gastric biopsy material taken from human immunodeficiency virus-infected subjects.

Authors:  Paolo Scarpellini; Paola Carrera; Annalisa Cavallero; Massimo Cernuschi; Gianni Mezzi; Pier Alberto Testoni; Anna Zingale; Adriano Lazzarin
Journal:  J Clin Microbiol       Date:  2002-06       Impact factor: 5.948

4.  Comparison of PCR with other diagnostic techniques for the detection of H. pylori infection in patients presenting with gastroduodenal symptons in Nigeria.

Authors:  Stella I Smith; Muinah A Fowora; Jesse A Otegbayo; Fatimah B Abdulkareem; Emmanuel A Omonigbehin; Akere Adegboyega; Monica Contreras; Rainer Haas
Journal:  Int J Mol Epidemiol Genet       Date:  2011-01-15

Review 5.  Molecular testing for antibiotic resistance in Helicobacter pylori.

Authors:  R J Owen
Journal:  Gut       Date:  2002-03       Impact factor: 23.059

6.  PCR-based diagnosis of Helicobacter pylori infection and real-time determination of clarithromycin resistance directly from human gastric biopsy samples.

Authors:  S A Chisholm; R J Owen; E L Teare; S Saverymuttu
Journal:  J Clin Microbiol       Date:  2001-04       Impact factor: 5.948
  6 in total

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