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Literature DB >> 9809068

DNA recognition and cleavage by the LAGLIDADG homing endonuclease I-CreI.

Abstract

The structure of the LAGLIDADG intron-encoded homing endonuclease I-CreI bound to homing site DNA has been determined. The interface is formed by an extended, concave beta sheet from each enzyme monomer that contacts each DNA half-site, resulting in direct side-chain contacts to 18 of the 24 base pairs across the full-length homing site. The structure indicates that I-CreI is optimized to its role in genetic transposition by exhibiting long site-recognition while being able to cleave many closely related target sequences. DNA cleavage is mediated by a compact pair of active sites in the I-CreI homodimer, each of which contains a separate bound divalent cation.

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Year: 1998 PMID： 9809068 DOI： 10.1016/s1097-2765(00)80146-x

Source DB: PubMed Journal: Mol Cell ISSN： 1097-2765 Impact factor: 17.970

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Cited

61 in total

1. Recruitment of intron-encoded and co-opted proteins in splicing of the bI3 group I intron RNA.

Authors: Gurminder S Bassi; Daniela M de Oliveira; Malcolm F White; Kevin M Weeks
Journal: Proc Natl Acad Sci U S A Date: 2002-01-02 Impact factor: 11.205

Review 2. Homing endonucleases: structural and functional insight into the catalysts of intron/intein mobility.

Authors: B S Chevalier; B L Stoddard
Journal: Nucleic Acids Res Date: 2001-09-15 Impact factor: 16.971

3. Unusual evolutionary history of the tRNA splicing endonuclease EndA: relationship to the LAGLIDADG and PD-(D/E)XK deoxyribonucleases.

Authors: J M Bujnicki; L Rychlewski
Journal: Protein Sci Date: 2001-03 Impact factor: 6.725

4. A novel engineered meganuclease induces homologous recombination in yeast and mammalian cells.

Authors: Jean-Charles Epinat; Sylvain Arnould; Patrick Chames; Pascal Rochaix; Dominique Desfontaines; Clémence Puzin; Amélie Patin; Alexandre Zanghellini; Frédéric Pâques; Emmanuel Lacroix
Journal: Nucleic Acids Res Date: 2003-06-01 Impact factor: 16.971

5. Structural and biochemical analyses of DNA and RNA binding by a bifunctional homing endonuclease and group I intron splicing factor.

Authors: Jill M Bolduc; P Clint Spiegel; Piyali Chatterjee; Kristina L Brady; Maureen E Downing; Mark G Caprara; Richard B Waring; Barry L Stoddard
Journal: Genes Dev Date: 2003-11-21 Impact factor: 11.361

DNA recognition and cleavage by the LAGLIDADG homing endonuclease I-CreI.

1. Recruitment of intron-encoded and co-opted proteins in splicing of the bI3 group I intron RNA.

Review 2. Homing endonucleases: structural and functional insight into the catalysts of intron/intein mobility.

3. Unusual evolutionary history of the tRNA splicing endonuclease EndA: relationship to the LAGLIDADG and PD-(D/E)XK deoxyribonucleases.

4. A novel engineered meganuclease induces homologous recombination in yeast and mammalian cells.

5. Structural and biochemical analyses of DNA and RNA binding by a bifunctional homing endonuclease and group I intron splicing factor.

6. Mutations altering the cleavage specificity of a homing endonuclease.

7. High resolution crystal structure of domain I of the Saccharomyces cerevisiae homing endonuclease PI-SceI.

8. Analysis of the LAGLIDADG interface of the monomeric homing endonuclease I-DmoI.

9. Homology modeling and mutational analysis of Ho endonuclease of yeast.

10. 5'-Cytosine-phosphoguanine (CpG) methylation impacts the activity of natural and engineered meganucleases.