J Hempstock1, J P Kavanagh, N J George. 1. Department of Urology, University Hospital of South Manchester, Withington, Manchester, UK.
Abstract
OBJECTIVE: To assess a range of phyto-oestrogens as moderators of growth and metabolism in several prostate cell lines. MATERIALS AND METHODS: Four prostate cell lines (PNT-1/A, PNT-2, PC-3 and DU145) were challenged with different doses of five phyto-oestrogens (biochanin A, daidzein, genistein, genistin and nordihydroguaiaretic acid) over 3 days in culture. Cell proliferation was assessed by incorporation of 5-bromo-2'-deoxyuridine (BrdU) and metabolic activity by cleavage of a tetrazolium salt (XTT). RESULTS: Growth and metabolism were inhibited with all compounds and cell lines (e.g. the dose for 50% inhibition of proliferation of PC-3 cells by genistein was 38 micromol/L); differences in the patterns of results suggested that different mechanisms operated, but there was no evidence for any synergistic activity on the inhibition of cell proliferation. CONCLUSION: These results offer further support for the hypothesized role of phyto-oestrogens as dietary protectors against prostatic cancer.
OBJECTIVE: To assess a range of phyto-oestrogens as moderators of growth and metabolism in several prostate cell lines. MATERIALS AND METHODS: Four prostate cell lines (PNT-1/A, PNT-2, PC-3 and DU145) were challenged with different doses of five phyto-oestrogens (biochanin A, daidzein, genistein, genistin and nordihydroguaiaretic acid) over 3 days in culture. Cell proliferation was assessed by incorporation of 5-bromo-2'-deoxyuridine (BrdU) and metabolic activity by cleavage of a tetrazolium salt (XTT). RESULTS: Growth and metabolism were inhibited with all compounds and cell lines (e.g. the dose for 50% inhibition of proliferation of PC-3 cells by genistein was 38 micromol/L); differences in the patterns of results suggested that different mechanisms operated, but there was no evidence for any synergistic activity on the inhibition of cell proliferation. CONCLUSION: These results offer further support for the hypothesized role of phyto-oestrogens as dietary protectors against prostatic cancer.
Authors: Z Weihua; S Makela; L C Andersson; S Salmi; S Saji; J I Webster; E V Jensen; S Nilsson; M Warner; J A Gustafsson Journal: Proc Natl Acad Sci U S A Date: 2001-05-22 Impact factor: 11.205