PURPOSE: This experiment was designed to help understand the cascade of events that end in renal impairment in septic animals. METHODS: Twenty 3- to 8-day-old piglets were anesthetized and the femoral artery, jugular and femoral veins, and bladder were catheterized. After stabilization under anesthesia with ventilatory support, they were divided into a control group and three groups that received endotoxin (ETX) in doses of 0.01 mg/kg, 0.025 mg/kg, and 0.05 mg/kg. Blood pressure and blood gases were monitored continuously. Blood and urine samples were obtained before (B), 1 hour (E1), and 3 hours (E3) after the bolus of ETX to determine glomerular filtration rate (GFR), fractional excretion of sodium (FENa), tumor necrosis factor (TNF), and endothelin-1 (ET-1) levels. RESULTS: Incremental doses of ETX induce greater release of ET-1 with an early proportionate increase in FENa (P< .05) and late decrease in GFR (P< .05). TNF release is dose and time dependent after ETX injection (P < .05). CONCLUSION: ET-1 and FENa are the best tests to evaluate renal failure during early sepsis in neonatal piglets.
PURPOSE: This experiment was designed to help understand the cascade of events that end in renal impairment in septic animals. METHODS: Twenty 3- to 8-day-old piglets were anesthetized and the femoral artery, jugular and femoral veins, and bladder were catheterized. After stabilization under anesthesia with ventilatory support, they were divided into a control group and three groups that received endotoxin (ETX) in doses of 0.01 mg/kg, 0.025 mg/kg, and 0.05 mg/kg. Blood pressure and blood gases were monitored continuously. Blood and urine samples were obtained before (B), 1 hour (E1), and 3 hours (E3) after the bolus of ETX to determine glomerular filtration rate (GFR), fractional excretion of sodium (FENa), tumor necrosis factor (TNF), and endothelin-1 (ET-1) levels. RESULTS: Incremental doses of ETX induce greater release of ET-1 with an early proportionate increase in FENa (P< .05) and late decrease in GFR (P< .05). TNF release is dose and time dependent after ETX injection (P < .05). CONCLUSION:ET-1 and FENa are the best tests to evaluate renal failure during early sepsis in neonatal piglets.
Authors: Kathryn A Seely; Joseph H Holthoff; Samuel T Burns; Zhen Wang; Keshari M Thakali; Neriman Gokden; Sung W Rhee; Philip R Mayeux Journal: Am J Physiol Renal Physiol Date: 2011-04-20
Authors: S V Demyttenaere; L S Feldman; S Bergman; S Gholoum; C Moriello; B Unikowsky; S Fraser; F Carli; G M Fried Journal: Surg Endosc Date: 2005-12-09 Impact factor: 4.584