BACKGROUND: The mechanisms involved in the initiation and the maintenance of skin inflammation in atopic dermatitis (AD) are poorly understood. Previous studies have demonstrated increased numbers of infiltrating CD4+ T cells in acute lesions compared with normal control skin. IL-16 is a cytokine that has selective chemotactic activity for CD4+ cells. OBJECTIVE: We sought to examine whether IL-16 expression might be upregulated in acute versus chronic AD. METHODS: We investigated the expression of IL-16 mRNA in skin biopsy specimens from acute and chronic skin lesions, as well as from the uninvolved skin of patients with AD and normal skin. Cryostat sections from 4% paraformaldehyde-fixed skin biopsy specimens were processed for in situ hybridization by using cRNA coding for IL-16 mRNA. Numbers of infiltrating CD4+ and CD8+ cells were also determined by immunocytochemistry. RESULTS: There were positive signals for IL-16 mRNA both in the basal layer of the epidermis and in the dermis of AD skin biopsy specimens from all subjects studied. The numbers of epidermal and dermal IL-16 mRNA+ cells were significantly increased in acute skin lesions compared with chronic (P <.01) and uninvolved (P <.001) skin lesions and compared with normal skin (P <.001). The number of CD4+ cells was significantly increased in acute skin lesions compared with chronic (P <.01) skin lesions and uninvolved skin (P <.01) and compared with normal skin (P <.01). Significant correlations were found between the numbers of CD4+ cells and the numbers of epidermal (r = 0.82, P <.001) and dermal (r = 0.71, P <.001) IL-16 mRNA+ cells. CONCLUSION: The results demonstrate that upregulation of IL-16 mRNA expression in acute AD is associated with increased numbers of CD4+ cells, suggesting that IL-16 may play a role in the initiation of skin inflammation, presumably through recruitment of CD4+ cells.
BACKGROUND: The mechanisms involved in the initiation and the maintenance of skin inflammation in atopic dermatitis (AD) are poorly understood. Previous studies have demonstrated increased numbers of infiltrating CD4+ T cells in acute lesions compared with normal control skin. IL-16 is a cytokine that has selective chemotactic activity for CD4+ cells. OBJECTIVE: We sought to examine whether IL-16 expression might be upregulated in acute versus chronic AD. METHODS: We investigated the expression of IL-16 mRNA in skin biopsy specimens from acute and chronic skin lesions, as well as from the uninvolved skin of patients with AD and normal skin. Cryostat sections from 4% paraformaldehyde-fixed skin biopsy specimens were processed for in situ hybridization by using cRNA coding for IL-16 mRNA. Numbers of infiltrating CD4+ and CD8+ cells were also determined by immunocytochemistry. RESULTS: There were positive signals for IL-16 mRNA both in the basal layer of the epidermis and in the dermis of AD skin biopsy specimens from all subjects studied. The numbers of epidermal and dermal IL-16 mRNA+ cells were significantly increased in acute skin lesions compared with chronic (P <.01) and uninvolved (P <.001) skin lesions and compared with normal skin (P <.001). The number of CD4+ cells was significantly increased in acute skin lesions compared with chronic (P <.01) skin lesions and uninvolved skin (P <.01) and compared with normal skin (P <.01). Significant correlations were found between the numbers of CD4+ cells and the numbers of epidermal (r = 0.82, P <.001) and dermal (r = 0.71, P <.001) IL-16 mRNA+ cells. CONCLUSION: The results demonstrate that upregulation of IL-16 mRNA expression in acute AD is associated with increased numbers of CD4+ cells, suggesting that IL-16 may play a role in the initiation of skin inflammation, presumably through recruitment of CD4+ cells.
Authors: Jian C Qi; Jing Wang; Sravan Mandadi; Kumiko Tanaka; Basil D Roufogalis; Michele C Madigan; Kenneth Lai; Feng Yan; Beng H Chong; Richard L Stevens; Steven A Krilis Journal: Blood Date: 2005-09-06 Impact factor: 22.113
Authors: Catherine A Thornton; Judith A Holloway; Janis K Shute; John W Holloway; Norma D Diaper; John O Warner Journal: Immunology Date: 2009-04 Impact factor: 7.397
Authors: Julia K Gittler; Avner Shemer; Mayte Suárez-Fariñas; Judilyn Fuentes-Duculan; Kara J Gulewicz; Claire Q F Wang; Hiroshi Mitsui; Irma Cardinale; Cristina de Guzman Strong; James G Krueger; Emma Guttman-Yassky Journal: J Allergy Clin Immunol Date: 2012-08-27 Impact factor: 10.793
Authors: Russell P Bowler; Timothy M Bahr; Grant Hughes; Sharon Lutz; Yu-Il Kim; Christopher D Coldren; Nichole Reisdorph; Katerina J Kechris Journal: OMICS Date: 2013-10-19
Authors: Yael Renert-Yuval; Ester Del Duca; Ana B Pavel; Milie Fang; Rachel Lefferdink; Jianni Wu; Aisleen Diaz; Yeriel D Estrada; Talia Canter; Ning Zhang; Annette Wagner; Sarah Chamlin; James G Krueger; Emma Guttman-Yassky; Amy S Paller Journal: J Allergy Clin Immunol Date: 2021-01-13 Impact factor: 14.290
Authors: Pablo Ramon Gualberto Cardoso; Claudia Diniz Lopes Marques; Kamila de Melo Vilar; Andrea Tavares Dantas; Angela Luzia Branco Pinto Duarte; Ivan da Rocha Pitta; Maira Galdino da Rocha Pitta; Moacyr Jesus Barreto de Melo Rêgo Journal: Autoimmune Dis Date: 2021-05-10