Literature DB >> 9801328

A rapid flow cytometric method to explore cellular immunity against Toxoplasma gondii in humans.

S Kahi1, G J Cozon, T Greenland, M Wallon, F Gay-Andrieu, F Peyron.   

Abstract

To assess cell-mediated immunity to Toxoplasma gondii, we evaluated the expression of the activation antigens CD69, CD71, and CD25 on T lymphocytes by flow cytometry after specific in vitro stimulation of whole blood from 127 T. gondii-positive and 63 T. gondii-negative patients. T lymphocytes from many seropositive individuals did not express CD69 at 24 h after T. gondii antigen stimulation, but CD71 and CD25 were easily detectable on T cells from seropositive individuals 7 days after specific activation. CD25 was mainly expressed by stimulated CD4(+) T cells, and its detection on total T cells was both a sensitive (98%) and a specific (97%) indicator of prior T. gondii infection. These results make flow cytometric detection of CD25 an excellent candidate for screening cell-mediated immunity to T. gondii in vitro and an interesting tool for the diagnosis of congenital infection.

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Year:  1998        PMID： 9801328      PMCID： PMC96195          DOI： 10.1128/CDLI.5.6.745-748.1998

Source DB:  PubMed          Journal:  Clin Diagn Lab Immunol        ISSN： 1071-412X


  15 in total

1.  Flow cytometric analysis of activation markers on stimulated T cells and their correlation with cell proliferation.

Authors:  A Caruso; S Licenziati; M Corulli; A D Canaris; M A De Francesco; S Fiorentini; L Peroni; F Fallacara; F Dima; A Balsari; A Turano
Journal:  Cytometry       Date:  1997-01-01

2.  Measurement of T-cell CD69 expression: a rapid and efficient means to assess mitogen- or antigen-induced proliferative capacity in normals.

Authors:  M Mardiney; M R Brown; T A Fleisher
Journal:  Cytometry       Date:  1996-12-15

3.  CD4-mediated and CD8-mediated cytotoxic and proliferative immune responses to Toxoplasma gondii in seropositive humans.

Authors:  M B Purner; R L Berens; P B Nash; A van Linden; E Ross; C Kruse; E C Krug; T J Curiel
Journal:  Infect Immun       Date:  1996-10       Impact factor: 3.441

4.  Cross-reactivity of human Toxoplasma-specific T cells: implications for development of a potential immunotherapeutic or vaccine.

Authors:  M B Purner; E C Krug; P Nash; D R Cook; R L Berens; T J Curiel
Journal:  J Infect Dis       Date:  1995-04       Impact factor: 5.226

5.  Chronological development of cellular immunity in human toxoplasmosis.

Authors:  W D Johnson
Journal:  Infect Immun       Date:  1981-09       Impact factor: 3.441

6.  Lymphocyte transformation in the diagnosis of congenital toxoplasma infection.

Authors:  C B Wilson; G Desmonts; J Couvreur; J S Remington
Journal:  N Engl J Med       Date:  1980-04-03       Impact factor: 91.245

7.  Longitudinal studies of lymphocyte response to Toxoplasma antigen in humans infected with T. gondii.

Authors:  S E Anderson; J L Krahenbuhl; J S Remington
Journal:  J Clin Lab Immunol       Date:  1979-11

8.  Infants potentially at risk for congenital toxoplasmosis: a prospective study.

Authors:  B Stray-Pedersen
Journal:  Am J Dis Child       Date:  1980-07

9.  Lymphocyte specificity to protein antigens. I. Characterization of the antigen-induced in vitro T cell-dependent proliferative response with lymph node cells from primed mice.

Authors:  G Corradin; H M Etlinger; J M Chiller
Journal:  J Immunol       Date:  1977-09       Impact factor: 5.422

10.  Toxoplasma gondii possesses a superantigen activity that selectively expands murine T cell receptor V beta 5-bearing CD8+ lymphocytes.

Authors:  E Y Denkers; P Caspar; A Sher
Journal:  J Exp Med       Date:  1994-09-01       Impact factor: 14.307
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Authors:  S Kahi; G J Cozon; J M Pinon; T Greenland; M Wallon; M Al Kurdi; J Ferrandiz; F Peyron
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4.  Cellular immunity to Toxoplasma gondii in congenitally infected newborns and immunocompetent infected hosts.

Authors:  A F Fatoohi; G J N Cozon; M Wallon; S Kahi; F Gay-Andrieu; T Greenland; F Peyron
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2003-03-04       Impact factor: 3.267

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6.  Heterogeneity in cellular and humoral immune responses against Toxoplasma gondii antigen in humans.

Authors:  A F Fatoohi; G J N Cozon; P Gonzalo; M Mayencon; T Greenland; S Picot; F Peyron
Journal:  Clin Exp Immunol       Date:  2004-06       Impact factor: 4.330

7.  Systemic T cell response to Toxoplasma gondii antigen in patients with ocular toxoplasmosis.

Authors:  Fatih Fatoohi; Grégoire Jacques Noël Cozon; Martine Wallon; Laurent Kodjikian; François Peyron
Journal:  Jpn J Ophthalmol       Date:  2006 Mar-Apr       Impact factor: 2.211

8.  Identification of Mycobacterium tuberculosis-specific Th1, Th17 and Th22 cells using the expression of CD40L in tuberculous pleurisy.

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9.  A new IgG immunoblot kit for diagnosis of toxoplasmosis in pregnant women.

Authors:  Imen Khammari; Fatma Saghrouni; Sami Lakhal; Aida Bouratbine; Moncef Ben Said; Jalel Boukadida
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