A Particle-Level Model of Irreversible Protein Adsorption with a Postadsorption Transition.

Modeling the kinetics of protein adsorption at solid surfaces is needed to predict protein separations, design biosensors, and determine the body's initial response to foreign objects. We develop, at the particle level, a kinetic model that accounts geometrically for the surface blockage due to adsorption and postadsorption conformational (or orientational) transitions. Proteins are modeled as disk-shaped particles of diameter final sigmaalpha that adsorb irreversibly at random positions onto a surface at a rate kac (c is the concentration of protein in the bulk solution). Adsorption occurs only where the surface is empty. Following adsorption, a particle attempts to spread (symmetrically) to a larger diameter final sigmabeta at a rate ks. Spreading only occurs if no overlap with any previously placed particle would result. A set of equations is developed for determining the time evolution of the adsorbed protein density. These predictions are compared to new experimental data for fibronectin onto silica-titania obtained using optical waveguide lightmode spectroscopy (OWLS). We also discuss the general application of this model to experimental data. Copyright 1998 Academic Press.