Apolipoprotein E and longevity among Han Chinese population.

The apolipoprotein E gene (APOE), which locates on chromosome 19, has three common APOE alleles encoding APOE*2, APOE*3, APOE*4. It has been considered to be closely associated with human longevity among Western population. However, the allele frequency varies among racial and ethnic groups. We studied the relationship between APOE and longevity among 1562 Han Chinese (246 female and 1316 male) whose ages ranged from 20-108 years old in Shanghai metropolitan area and Lian Yungang, Jiangsu province. APOE was amplified by one-stage PCR using the forward primer: 5'-GGC ACG GCT GTC CAA GGA GCT-3' and reverse primer: 5'-GAT GGC GCT GAG GCC GCG CT-3'. The PCR product was digested directly with 5 U of CfoI and separated by a 20% polyacrylamide (acrylamide:bisacrylamide = 29:1) nondenaturing gel. The allele frequency of APOE*4 in thevery old age group (> or = 85 years) was significantly different from that in the youth (20-39 years), middle (40-59 years) and old (60-84 years) age group (2.5% vs 8.4, 7.9 or 7.6%; P < 0.05), respectively.