OBJECTIVE: To characterize and compare the expression of PECAM-1 in unstimulated and tumor necrosis factor alpha-(TNF-alpha)-stimulated tissues of mice. METHODS: Binding and non-binding monoclonal antibodies (mAb) were radiolabeled and injected into wild-type mice and mice deficient of P-selectin, CD18, or ICAM-1. The relative accumulation of binding mAb (PECAM-1 mAb) was determined in wild-type mice following a 25 micrograms/kg i.p. injection of TNF-alpha and in mutant mice under basal conditions. RESULTS: Under unstimulated conditions, PECAM-1 was significantly expressed in all tissues examined, with no changes occurring after TNF-alpha stimulation. An equivalence of PECAM-1 expression was observed in unstimulated tissues among wild-type mice and mice that are genetically deficient in either CD18, ICAM-1, or P-selectin. The level of PECAM-1 expression in different vascular beds was highly correlated to published values of endothelial surface area. Normalization of previously published values of ICAM-1, VCAM-1, E- and P-selectin expression relative to PECAM-1 expression in the same tissues revealed a diminished organ-to-organ variability in expression of the different adhesion molecules. Estimates of adhesion molecule expression in lung and brain were most profoundly affected by normalization to PECAM-1 expression. CONCLUSIONS: These findings support the use of PECAM-1 expression in regional vascular beds as an indicator of endothelial cell surface area.
OBJECTIVE: To characterize and compare the expression of PECAM-1 in unstimulated and tumor necrosis factor alpha-(TNF-alpha)-stimulated tissues of mice. METHODS: Binding and non-binding monoclonal antibodies (mAb) were radiolabeled and injected into wild-type mice and mice deficient of P-selectin, CD18, or ICAM-1. The relative accumulation of binding mAb (PECAM-1 mAb) was determined in wild-type mice following a 25 micrograms/kg i.p. injection of TNF-alpha and in mutant mice under basal conditions. RESULTS: Under unstimulated conditions, PECAM-1 was significantly expressed in all tissues examined, with no changes occurring after TNF-alpha stimulation. An equivalence of PECAM-1 expression was observed in unstimulated tissues among wild-type mice and mice that are genetically deficient in either CD18, ICAM-1, or P-selectin. The level of PECAM-1 expression in different vascular beds was highly correlated to published values of endothelial surface area. Normalization of previously published values of ICAM-1, VCAM-1, E- and P-selectin expression relative to PECAM-1 expression in the same tissues revealed a diminished organ-to-organ variability in expression of the different adhesion molecules. Estimates of adhesion molecule expression in lung and brain were most profoundly affected by normalization to PECAM-1 expression. CONCLUSIONS: These findings support the use of PECAM-1 expression in regional vascular beds as an indicator of endothelial cell surface area.
Authors: Vanessa Nomellini; Aleah L Brubaker; Shegufta Mahbub; Jessica L Palmer; Christian R Gomez; Elizabeth J Kovacs Journal: Aging Dis Date: 2012-02-28 Impact factor: 6.745
Authors: John H Chidlow; Will Langston; James J M Greer; Dmitry Ostanin; Maisoun Abdelbaqi; Jeffery Houghton; Annamalai Senthilkumar; Deepti Shukla; Andrew P Mazar; Matthew B Grisham; Christopher G Kevil Journal: Am J Pathol Date: 2006-12 Impact factor: 4.307
Authors: F J Giordano; H P Gerber; S P Williams; N VanBruggen; S Bunting; P Ruiz-Lozano; Y Gu; A K Nath; Y Huang; R Hickey; N Dalton; K L Peterson; J Ross; K R Chien; N Ferrara Journal: Proc Natl Acad Sci U S A Date: 2001-05-01 Impact factor: 11.205
Authors: John Street; Min Bao; Leo deGuzman; Stuart Bunting; Franklin V Peale; Napoleone Ferrara; Hope Steinmetz; John Hoeffel; Jeffrey L Cleland; Ann Daugherty; Nicholas van Bruggen; H Paul Redmond; Richard A D Carano; Ellen H Filvaroff Journal: Proc Natl Acad Sci U S A Date: 2002-07-12 Impact factor: 11.205
Authors: Daniela Tirziu; Emmanuel Chorianopoulos; Karen L Moodie; Robert T Palac; Zhen W Zhuang; Marc Tjwa; Carmen Roncal; Ulf Eriksson; Qiangwei Fu; Arye Elfenbein; Amy E Hall; Peter Carmeliet; Lieve Moons; Michael Simons Journal: J Clin Invest Date: 2007-11 Impact factor: 14.808