| Literature DB >> 9787771 |
C Caruso-Neves1, J R Meyer-Fernandes, J Saad-Nehme, F Proverbio, R Marín, A G Lopes.
Abstract
In the present paper, we show the existence of a furosemide-sensitive Na(+)-stimulated, Mg(2+)-dependent ATPase activity in cell lysates of Malpighian tubular cells from Rhodnius prolixus, which could be the biochemical expression of the Na(+)-pump. The main characteristics of this activity are: (1) K0.5 for Na+ = 1.49 +/- 0.18 mM, (2) Vmax = 2.8 +/- 0.1 nmol inorganic orthophosphate (Pi).mg prot-1.min-1, (3) it is fully abolished by 2 mM furosemide, (4)it is insensitive to ouabain concentrations up to 10(-2) M, (5) it is sensitive to the presence of vanadate in the incubation medium indicating it to be a P-type ATPase, and (6) it is stimulated by nanomolar concentrations of Ca2+ in the incubation medium.Entities:
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Year: 1998 PMID： 9787771 DOI： 10.1016/s0305-0491(98)00057-1
Source DB: PubMed Journal: Comp Biochem Physiol B Biochem Mol Biol ISSN： 1096-4959 Impact factor: 2.231