BACKGROUND AND OBJECTIVES: Significant amounts of di(2-ethylhexyl) phthlate (DEHP) leach into blood stored in DEHP-plasticized PVC bags. The aim of this study was to find out whether DEHP at these low levels has any effect on the concentration of vitamin E, an antioxidant which affords protection against free radical damage. MATERIALS AND METHODS: DEHP was administered in low doses (150-750 microg/100 g body weight) to rats intraperitoneally and the concentration of vitamin E in the liver and testes was measured. Concentration of vitamin E was also measured in blood stored in glass bottles in the presence and absence of DEHP and in blood stored in DEHP-plasticized PVC bags. RESULTS: A decrease in the concentration of vitamin E was observed in all cases. Administration of vitamin E to rats and incorporation of vitamin E in the additive solution in the case of blood prevented this decrease. CONCLUSION: DEHP even at very low doses caused a decrease in the concentration of vitamin E in liver and tests of rats given this substance. Blood stored in DEHP-plasticized bags also showed a decrease in the concentration of vitamin E.
BACKGROUND AND OBJECTIVES: Significant amounts of di(2-ethylhexyl) phthlate (DEHP) leach into blood stored in DEHP-plasticized PVC bags. The aim of this study was to find out whether DEHP at these low levels has any effect on the concentration of vitamin E, an antioxidant which affords protection against free radical damage. MATERIALS AND METHODS:DEHP was administered in low doses (150-750 microg/100 g body weight) to rats intraperitoneally and the concentration of vitamin E in the liver and testes was measured. Concentration of vitamin E was also measured in blood stored in glass bottles in the presence and absence of DEHP and in blood stored in DEHP-plasticized PVC bags. RESULTS: A decrease in the concentration of vitamin E was observed in all cases. Administration of vitamin E to rats and incorporation of vitamin E in the additive solution in the case of blood prevented this decrease. CONCLUSION:DEHP even at very low doses caused a decrease in the concentration of vitamin E in liver and tests of rats given this substance. Blood stored in DEHP-plasticized bags also showed a decrease in the concentration of vitamin E.
Authors: Barbara Ambruosi; Manuel Filioli Uranio; Anna Maria Sardanelli; Paola Pocar; Nicola Antonio Martino; Maria Stefania Paternoster; Francesca Amati; Maria Elena Dell'Aquila Journal: PLoS One Date: 2011-11-04 Impact factor: 3.240