| Literature DB >> 9784069 |
T Yamamoto1, S Suzuki, K Radsak, K Hirai.
Abstract
The UL112/113 gene products of human cytomegalovirus (HCMV) were shown by transient complementation ori Lyt-dependent DNA replication assay to be early viral proteins required for efficient viral DNA synthesis. By immunofluorescence analysis followed by fluorescence in situ hybridization, we showed that UL112/113 gene products of HCMV are colocalized with viral DNA prior to and during viral DNA replication in infected cell nuclei. We have used an anti-sense RNA approach for functional analysis of the UL112/113 gene in HCMV. The astrocytoma cell line U373-MG was used for permanent expression of the anti-sense UL112/113 gene. Expression of the anti-sense RNA in this cell line significantly blocked expression of UL112/113 gene products and viral DNA replication, indicating that the UL112/113 gene products are related to efficient viral DNA replication.Entities:
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Year: 1998 PMID： 9784069 DOI： 10.1016/s0168-1702(98)00032-x
Source DB: PubMed Journal: Virus Res ISSN： 0168-1702 Impact factor: 3.303