Literature DB >> 9780736

[RAPD typing of Campylobacter jejuni and comparison with Lior's or Penner's serotyping system].

K Ono1, K Yamamoto, A Niwa.   

Abstract

Campylobacter jejuni were isolated from 7 epidemic outbreaks (121 isolates), 15 patients with gastroenteritis, chicken meats (47 isolates) and chicken cecal contents (70 isolates). The isolates and one standard strain of C. jejuni JCM2013 were analysed by randomly amplified polymorphic DNA method (RAPD). Total of 254 C. jejuni isolates were divided 68 different RAPD types which included strains that did not to divided by Lior's or Penner's serotyping system. To compare the similarities of RAPD patterns among the isolates, the amplification patterns of DNA were estimated by means of the Dice coefficient, and clustering of strains was based on the unweighted average pair group method (UPGMA) to facilitate the plotting of a dendrogram. It suggests that amplification band patterns of human isolates were different from those of chicken ones. Thus additional information given from RAPD profiles serves for epidemiological investigation and RAPD analysis is recommended as rapid and effective typing method.

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Year:  1998        PMID: 9780736     DOI: 10.3412/jsb.53.519

Source DB:  PubMed          Journal:  Nihon Saikingaku Zasshi        ISSN: 0021-4930


  1 in total

1.  Effect of incubation temperature on isolation of Campylobacter jejuni genotypes from foodstuffs enriched in Preston broth.

Authors:  Pam Scates; Lynn Moran; Robert H Madden
Journal:  Appl Environ Microbiol       Date:  2003-08       Impact factor: 4.792

  1 in total

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