OBJECTIVE: To determine the effect of subcutaneous (s.c.) as compared to intradermal (i.d.) inoculation of collagen type II (CII) in induction of collagen induced arthritis (CIA). METHODS: Dark Agouti (DA) and Lewis rats were injected with CII either i.d. or s.c.. A group of s.c. inoculated DA rats was re-injected with CII intradermally 45 days after first injection (s.c./i.d.). Arthritis was assessed by macroscopic scoring, histology, and immunohistochemistry. Levels of anti-CII antibody subtypes were measured by ELISA. RESULTS: Intradermal but not s.c. inoculation of CII resulted in histologically confirmed erosive arthritis in both Lewis and DA strains. Subcutaneous/intradermal inoculated DA rats developed mild CIA with lower arthritic scores and delayed onset. Lewis rats injected s.c. had lower levels of total Ig, IgG, IgG2a, and IgG2b and similar titers of IgG1 compared to i.d. inoculated rats. In contrast, only IgG2b levels were lower in s.c./i.d. compared to i.d. rats. CONCLUSION: Our data suggest that s.c. administration of CII tolerises animals against autoimmune CIA.
OBJECTIVE: To determine the effect of subcutaneous (s.c.) as compared to intradermal (i.d.) inoculation of collagen type II (CII) in induction of collagen induced arthritis (CIA). METHODS: Dark Agouti (DA) and Lewis rats were injected with CII either i.d. or s.c.. A group of s.c. inoculated DA rats was re-injected with CII intradermally 45 days after first injection (s.c./i.d.). Arthritis was assessed by macroscopic scoring, histology, and immunohistochemistry. Levels of anti-CII antibody subtypes were measured by ELISA. RESULTS: Intradermal but not s.c. inoculation of CII resulted in histologically confirmed erosive arthritis in both Lewis and DA strains. Subcutaneous/intradermal inoculated DA rats developed mild CIA with lower arthritic scores and delayed onset. Lewis rats injected s.c. had lower levels of total Ig, IgG, IgG2a, and IgG2b and similar titers of IgG1 compared to i.d. inoculated rats. In contrast, only IgG2b levels were lower in s.c./i.d. compared to i.d. rats. CONCLUSION: Our data suggest that s.c. administration of CII tolerises animals against autoimmune CIA.
Authors: Evan Romas; Natalie A Sims; Daphne K Hards; Mandy Lindsay; Julian W M Quinn; Peter F J Ryan; Colin R Dunstan; T John Martin; Matthew T Gillespie Journal: Am J Pathol Date: 2002-10 Impact factor: 4.307
Authors: Dina Shahin; Ehab El Toraby; Hala Abdel-Malek; Vivian Boshra; Ayman Z Elsamanoudy; Dalia Shaheen Journal: Clin Med Insights Arthritis Musculoskelet Disord Date: 2011-02-07