| Literature DB >> 977686 |
Abstract
An ion-exchange chromatographic method for the quantitative determination of glucosamine and galactosamine in glycoproteins is described. The hexosamines are completely separated from interfering peptides of the acid hydrolysate using a small column of cation exchanger in a manner described earlier by Boas. Chromatographic separation of the hexosamine fraction and the determination of glucosamine and galactosamine are accomplished on an amino acid analyzer with ninhydrin.Entities:
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Year: 1976 PMID: 977686 DOI: 10.1016/s0021-9673(00)83373-7
Source DB: PubMed Journal: J Chromatogr