Literature DB >> 9774559

Diagnostic potential of Toscana virus N protein expressed in Escherichia coli.

M Valassina1, D Soldateschi, G M Dal Maso, L Santini, S Bianchi, P E Valensin, M G Cusi.   

Abstract

The nucleocapsid (N) protein of the Toscana (TOS) virus was expressed in Escherichia coli by using a pET15b vector. The recombinant protein was purified by affinity chromatography and was characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, immunoblotting, and enzyme immunoassay (EIA). The recombinant antigen was reactive with positive human sera, and the reactivity correlated very well (r = 0.9) with that of a whole-virus antigen when tested by EIA with 30 TOS virus-positive and 30 TOS virus-negative serum samples. The results demonstrate that the recombinant N protein can be easily produced in a procaryotic system and used for diagnostic assays for TOS virus immunity.

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Year:  1998        PMID： 9774559      PMCID： PMC105295     

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  21 in total

1.  Analysis of 3' and 5' ends of N and NSs messenger RNAs of Toscana Phlebovirus.

Authors:  M C Grò; P Di Bonito; L Accardi; C Giorgi
Journal:  Virology       Date:  1992-11       Impact factor: 3.616

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Journal:  Virus Res       Date:  1991-03       Impact factor: 3.303

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Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

5.  Neurovirulent Toscana virus (a sandfly fever virus) in Swedish man after visit to Portugal.

Authors:  A Ehrnst; C J Peters; B Niklasson; A Svedmyr; B Holmgren
Journal:  Lancet       Date:  1985-05-25       Impact factor: 79.321

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Authors:  P Verani; L Nicoletti; M G Ciufolini; M Balducci
Journal:  Parassitologia       Date:  1991-12

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Journal:  Bull World Health Organ       Date:  1976       Impact factor: 9.408

8.  Toscana virus genomic L segment: molecular cloning, coding strategy and amino acid sequence in comparison with other negative strand RNA viruses.

Authors:  L Accardi; M C Grò; P Di Bonito; C Giorgi
Journal:  Virus Res       Date:  1993-02       Impact factor: 3.303

9.  Central nervous system involvement during infection by Phlebovirus toscana of residents in natural foci in central Italy (1977-1988).

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Journal:  Am J Trop Med Hyg       Date:  1991-10       Impact factor: 2.345

10.  Sandfly fever among Swedish tourists.

Authors:  R Eitrem; B Niklasson; O Weiland
Journal:  Scand J Infect Dis       Date:  1991
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  7 in total

1.  Serological survey of Toscana virus infections in a high-risk population in Italy.

Authors:  Marcello Valassina; Melissa Valentini; Agostino Pugliese; Pier Egisto Valensin; Maria Grazia Cusi
Journal:  Clin Diagn Lab Immunol       Date:  2003-05

2.  Granada virus: a natural phlebovirus reassortant of the sandfly fever Naples serocomplex with low seroprevalence in humans.

Authors:  Ximena Collao; Gustavo Palacios; Fernando de Ory; Sara Sanbonmatsu; Mercedes Pérez-Ruiz; José María Navarro; Ricardo Molina; Stephen K Hutchison; W Ian Lipkin; Antonio Tenorio; María Paz Sánchez-Seco
Journal:  Am J Trop Med Hyg       Date:  2010-10       Impact factor: 2.345

3.  Diagnosis of Oropouche virus infection using a recombinant nucleocapsid protein-based enzyme immunoassay.

Authors:  M F Saeed; M Nunes; P F Vasconcelos; A P Travassos Da Rosa; D M Watts; K Russell; R E Shope; R B Tesh; A D Barrett
Journal:  J Clin Microbiol       Date:  2001-07       Impact factor: 5.948

4.  Performance of various commercial assays for the detection of Toscana virus antibodies.

Authors:  Koray Ergünay; Nadine Litzba; Modou Moustapha Lo; Sibel Aydoğan; Mehmet B Saygan; Dürdal Us; Manfred Weidmann; Matthias Niedrig
Journal:  Vector Borne Zoonotic Dis       Date:  2011-03-11       Impact factor: 2.133

5.  Laboratory diagnosis of Toscana virus infection by enzyme immunoassay with recombinant viral nucleoprotein.

Authors:  D Soldateschi; G M dal Maso; M Valassina; L Santini; S Bianchi; M G Cusi
Journal:  J Clin Microbiol       Date:  1999-03       Impact factor: 5.948

Review 6.  A Mediterranean arbovirus: the Toscana virus.

Authors:  Marcello Valassina; Maria Grazia Cusi; Pier Egisto Valensin
Journal:  J Neurovirol       Date:  2003-12       Impact factor: 2.643

7.  Toscana virus non-structural protein NSs acts as E3 ubiquitin ligase promoting RIG-I degradation.

Authors:  Gianni Gori Savellini; Gabriele Anichini; Claudia Gandolfo; Shibily Prathyumnan; Maria Grazia Cusi
Journal:  PLoS Pathog       Date:  2019-12-09       Impact factor: 6.823
  7 in total

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