H L Ji1, T Y Wang. 1. Beijing Neurosurgical Institute, China.
Abstract
AIM: To test if puerarin (Pue) affects slow sodium current in dorsal root ganglion (DRG) neurons. METHODS: Tetrodotoxin resistant (TTXr) sodium current was recorded with whole cell patch clamp technique on DRG neurons of young adult rats. RESULTS: Pue 0.01-2 mmol.L-1 inhibited TTXr sodium current by 9.5%-83.2%. The inhibition was concentration-dependent and partially reversible, but was not use-dependent nor voltage-dependent. Pue did not affect the inactivation but changed the potential for half maximal conductance from -26 mV to -16 mV, suggesting the activation process was inhibited. CONCLUSION: Pue moderately inhibits TTXr sodium current of rat DRG neurons.
AIM: To test if puerarin (Pue) affects slow sodium current in dorsal root ganglion (DRG) neurons. METHODS:Tetrodotoxin resistant (TTXr) sodium current was recorded with whole cell patch clamp technique on DRG neurons of young adult rats. RESULTS:Pue 0.01-2 mmol.L-1 inhibited TTXr sodium current by 9.5%-83.2%. The inhibition was concentration-dependent and partially reversible, but was not use-dependent nor voltage-dependent. Pue did not affect the inactivation but changed the potential for half maximal conductance from -26 mV to -16 mV, suggesting the activation process was inhibited. CONCLUSION:Pue moderately inhibits TTXr sodium current of rat DRG neurons.