P Lan1, W Zhan, J Wang. 1. Department of Surgery, First Affiliated Hospital, West China University of Medical Sciences, Cheng.
Abstract
OBJECTIVE: To induce specific mouse to rat xenograft tolerance. METHODS: The antibodies affinity chromatography was used to purify mouse major histocompatibility complex antigens (H-2 Ag). H-2 Ag was conjugated with trichosanthin (TCS) by heterobifunctional reagents SPDP and 2-IT. The specific inhibition function of H-2 Ag-TCS to recipient immune cell was Measured. H-2Ag-TCS was used in mouse to rat cardiac xenotransplantation (cuff technique). RESULTS: The proliferation of recipient immune cell pretreated with conjugate H-2Ag-TCS was inhibited. H-2 Ag-TCS significanlly prolonged the cardiac survival time (6.88 +/- 1.36) compared with CgA group (2.83 +/- 0.75) (P < 0.01). CONCLUSION: H-2 Ag-TCS can induce specific tolerance to xenograft.
OBJECTIVE: To induce specific mouse to rat xenograft tolerance. METHODS: The antibodies affinity chromatography was used to purify mouse major histocompatibility complex antigens (H-2 Ag). H-2 Ag was conjugated with trichosanthin (TCS) by heterobifunctional reagents SPDP and 2-IT. The specific inhibition function of H-2 Ag-TCS to recipient immune cell was Measured. H-2Ag-TCS was used in mouse to rat cardiac xenotransplantation (cuff technique). RESULTS: The proliferation of recipient immune cell pretreated with conjugate H-2Ag-TCS was inhibited. H-2 Ag-TCS significanlly prolonged the cardiac survival time (6.88 +/- 1.36) compared with CgA group (2.83 +/- 0.75) (P < 0.01). CONCLUSION: H-2 Ag-TCS can induce specific tolerance to xenograft.