| Literature DB >> 9767106 |
Abstract
We describe an updated protocol which allows the combination of the previously described TUNEL and Hoechst 33342 double-labelling (G. Whiteside, N. Cougnon, S.P. Hunt, R. Munglani, An improved method for the detection of apoptosis in tissue sections and cell culture, using the TUNEL technique combined with Hoechst stain, Brain Res. Protocols, 2, 1998, 160-164 [3]), with immunohistochemistry. The inclusion of the immunohistochemistry step allows identification of apoptosis in distinct cell types by using cell specific markers. It also permits investigation into the proteins expressed in cells undergoing apoptotic cell death. We have evaluated the triple staining in sections of post-axotomy neonatal dorsal root ganglia. Copyright 1998 Elsevier Science B.V.Entities:
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Year: 1998 PMID： 9767106 DOI： 10.1016/s1385-299x(98)00020-8
Source DB: PubMed Journal: Brain Res Brain Res Protoc ISSN： 1385-299X