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Literature DB >> 9765582

Autoregulation of the pTF-FC2 proteic poison-antidote plasmid addiction system (pas) is essential for plasmid stabilization.

Abstract

The pasABC genes of the proteic plasmid addiction system of broad-host-range plasmid pTF-FC2 were autoregulated. The PasA antidote was able to repress the operon 25-fold on its own, and repression was increased to 100-fold when the PasB toxin was also present. Autoregulation appears to be an essential requirement for pas-mediated plasmid stabilization because when the pas genes were placed behind the isopropyl-beta-D-thiogalactopyranoside (IPTG)-regulated tac promoter, they were unable to stabilize a heterologous test plasmid.

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Year: 1998 PMID： 9765582 PMCID： PMC107599

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

14 in total

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Authors: A C Chang; S N Cohen
Journal: J Bacteriol Date: 1978-06 Impact factor: 3.490

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Journal: Gene Date: 1991-12-01 Impact factor: 3.688

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Journal: J Bacteriol Date: 1980-08 Impact factor: 3.490

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Authors: J E Tam; B C Kline
Journal: Mol Gen Genet Date: 1989-10

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Journal: J Bacteriol Date: 1985-01 Impact factor: 3.490

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Journal: Gene Date: 1985 Impact factor: 3.688

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Authors: R C Roberts; C Spangler; D R Helinski
Journal: J Biol Chem Date: 1993-12-25 Impact factor: 5.157

9. Efficiency of the pTF-FC2 pas poison-antidote stability system in Escherichia coli is affected by the host strain, and antidote degradation requires the lon protease.

Authors: A S Smith; D E Rawlings
Journal: J Bacteriol Date: 1998-10 Impact factor: 3.490

10. Regulation of ribosomal RNA promoters with a synthetic lac operator.

Authors: J Brosius; A Holy
Journal: Proc Natl Acad Sci U S A Date: 1984-11 Impact factor: 11.205

9 in total

Review 1. Toxin-antitoxin modules may regulate synthesis of macromolecules during nutritional stress.

Authors: K Gerdes
Journal: J Bacteriol Date: 2000-02 Impact factor: 3.490

2. Percolation of the phd repressor-operator interface.

Authors: Xueyan Zhao; Roy David Magnuson
Journal: J Bacteriol Date: 2005-03 Impact factor: 3.490

3. The toxin-antitoxin system of the streptococcal plasmid pSM19035.

Authors: Urszula Zielenkiewicz; Piotr Ceglowski
Journal: J Bacteriol Date: 2005-09 Impact factor: 3.490

4. Efficiency of the pTF-FC2 pas poison-antidote stability system in Escherichia coli is affected by the host strain, and antidote degradation requires the lon protease.