Literature DB >> 9765490

Identification and characterization of an RNA-dependent RNA polymerase activity within the nonstructural protein 5B region of bovine viral diarrhea virus.

W Zhong1, L L Gutshall, A M Del Vecchio.   

Abstract

Nonstructural protein 5B (NS5B) of bovine viral diarrhea virus (BVDV) contains sequence motifs that are predictive of an RNA-dependent RNA polymerase activity. We describe the expression and purification of the BVDV NS5B protein derived from an infectious cDNA clone of BVDV (NADL strain). BVDV NS5B protein was active in an in vitro RNA polymerase assay using homopolymeric RNA or BVDV minigenomic RNA templates. The major product was a covalently linked double-stranded molecule generated by a "copy-back" mechanism from the input template RNA. In addition, a nucleotide-nonspecific and template-independent terminal nucleotidyl transferase activity was observed with the BVDV NS5B preparation.

Entities:  

Mesh：

Substances：

Year:  1998        PMID： 9765490      PMCID： PMC110362     

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  20 in total

Review 1.  Molecular genetics of pestiviruses.

Authors:  M S Collett
Journal:  Comp Immunol Microbiol Infect Dis       Date:  1992-07       Impact factor: 2.268

Review 2.  The pestiviruses.

Authors:  V Moennig; P G Plagemann
Journal:  Adv Virus Res       Date:  1992       Impact factor: 9.937

Review 3.  Bovine viral diarrhea virus: a review.

Authors:  J C Baker
Journal:  J Am Vet Med Assoc       Date:  1987-06-01       Impact factor: 1.936

4.  5' and 3' untranslated regions of pestivirus genome: primary and secondary structure analyses.

Authors:  R Deng; K V Brock
Journal:  Nucleic Acids Res       Date:  1993-04-25       Impact factor: 16.971

5.  The P gene product of hepatitis B virus is required as a structural component for genomic RNA encapsidation.

Authors:  R Bartenschlager; M Junker-Niepmann; H Schaller
Journal:  J Virol       Date:  1990-11       Impact factor: 5.103

6.  Baculovirus expression of pestivirus non-structural proteins.

Authors:  M Petric; R H Yolken; E J Dubovi; M Wiskerchen; M S Collett
Journal:  J Gen Virol       Date:  1992-07       Impact factor: 3.891

7.  Processing of the envelope glycoproteins of pestiviruses.

Authors:  T Rümenapf; G Unger; J H Strauss; H J Thiel
Journal:  J Virol       Date:  1993-06       Impact factor: 5.103

8.  Molecular cloning and nucleotide sequence of the genome of hog cholera virus.

Authors:  G Meyers; T Rümenapf; H J Thiel
Journal:  Virology       Date:  1989-08       Impact factor: 3.616

9.  Proteins encoded by bovine viral diarrhea virus: the genomic organization of a pestivirus.

Authors:  M S Collett; R Larson; S K Belzer; E Retzel
Journal:  Virology       Date:  1988-07       Impact factor: 3.616

10.  Biochemical properties of hepatitis C virus NS5B RNA-dependent RNA polymerase and identification of amino acid sequence motifs essential for enzymatic activity.

Authors:  V Lohmann; F Körner; U Herian; R Bartenschlager
Journal:  J Virol       Date:  1997-11       Impact factor: 5.103
View more
  51 in total

1.  Factors regulating template switch in vitro by viral RNA-dependent RNA polymerases: implications for RNA-RNA recombination.

Authors:  M J Kim; C Kao
Journal:  Proc Natl Acad Sci U S A       Date:  2001-04-17       Impact factor: 11.205

2.  Use of DNA, RNA, and chimeric templates by a viral RNA-dependent RNA polymerase: evolutionary implications for the transition from the RNA to the DNA world.

Authors:  R W Siegel; L Bellon; L Beigelman; C C Kao
Journal:  J Virol       Date:  1999-08       Impact factor: 5.103

Review 3.  Perspectives for the treatment of infections with Flaviviridae.

Authors:  P Leyssen; E De Clercq; J Neyts
Journal:  Clin Microbiol Rev       Date:  2000-01       Impact factor: 26.132

4.  Template nucleotide moieties required for de novo initiation of RNA synthesis by a recombinant viral RNA-dependent RNA polymerase.

Authors:  M J Kim; W Zhong; Z Hong; C C Kao
Journal:  J Virol       Date:  2000-11       Impact factor: 5.103

5.  Mutations in the 5' nontranslated region of bovine viral diarrhea virus result in altered growth characteristics.

Authors:  P Becher; M Orlich; H J Thiel
Journal:  J Virol       Date:  2000-09       Impact factor: 5.103

6.  A cellular J-domain protein modulates polyprotein processing and cytopathogenicity of a pestivirus.

Authors:  G Rinck; C Birghan; T Harada; G Meyers; H J Thiel; N Tautz
Journal:  J Virol       Date:  2001-10       Impact factor: 5.103

7.  CRISPR/Cas9-Mediated Knockout of DNAJC14 Verifies This Chaperone as a Pivotal Host Factor for RNA Replication of Pestiviruses.

Authors:  O Isken; A Postel; B Bruhn; E Lattwein; P Becher; N Tautz
Journal:  J Virol       Date:  2019-02-19       Impact factor: 5.103

8.  Modification of the 5' terminus of Sindbis virus genomic RNA allows nsP4 RNA polymerases with nonaromatic amino acids at the N terminus to function in RNA replication.

Authors:  Yukio Shirako; Ellen G Strauss; James H Strauss
Journal:  J Virol       Date:  2003-02       Impact factor: 5.103

9.  Sensitivity of the polymerase of vesicular stomatitis virus to 2' substitutions in the template and nucleotide triphosphate during initiation and elongation.

Authors:  Benjamin Morin; Sean P J Whelan
Journal:  J Biol Chem       Date:  2014-02-13       Impact factor: 5.157

10.  Isolation and characterization of noncytopathic pestivirus mutants reveals a role for nonstructural protein NS4B in viral cytopathogenicity.

Authors:  L Qu; L K McMullan; C M Rice
Journal:  J Virol       Date:  2001-11       Impact factor: 5.103
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.