Literature DB >> 976436

Kinetics of lymphocyte division in blood cultures studied with the BrdU-Giemsa technique.

N O Bianchi, E A Lezana.   

Abstract

Normal human lymphocytes were cultured for 72 h with different doses of BrdU. The analysis of metaphases processed with the BrdU-Giemsa method shows that in leukocyte cultures 3 different lymphocyte populations coexist which are able to perform 1, 2 or 3 rounds of replication in vitro. Moreover, it was concluded that 5 mug/ml is the minimal dose of BrdU inducing good differentiation in the areas of sister chromatid exchanges.

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Year:  1976        PMID： 976436     DOI： 10.1007/BF01953080

Source DB:  PubMed          Journal:  Experientia        ISSN： 0014-4754


  9 in total

1.  Chromosome aberrations and T-cell survival in human lymphocytes.

Authors:  R Santos Mello; D Kwan; A Norman
Journal:  Radiat Res       Date:  1974-12       Impact factor: 2.841

2.  TIME-COURSE OF DNA SYNTHESIS IN HUMAN LEUKOCYTE CULTURES.

Authors:  A MICHALOWSKI
Journal:  Exp Cell Res       Date:  1963-12       Impact factor: 3.905

3.  The human leukocyte test system. I. DNA synthesis and mitoses in PHA-stimulated 2-day cultures.

Authors:  G Dudin; B Beek; G Obe
Journal:  Mutat Res       Date:  1974-05       Impact factor: 2.433

4.  Microfluorometric detection of deoxyribonucleic acid replication in human metaphase chromosomes.

Authors:  S A Latt
Journal:  Proc Natl Acad Sci U S A       Date:  1973-12       Impact factor: 11.205

5.  Factors influencing chromosome aberration yields in the human peripheral leukocyte system.

Authors:  M A Bender; J G Brewen
Journal:  Mutat Res       Date:  1969 Sep-Oct       Impact factor: 2.433

6.  Giemsa technique for the detection of sister chromatid exchanges.

Authors:  J R Korenberg; E F Freedlender
Journal:  Chromosoma       Date:  1974       Impact factor: 4.316

7.  Differential Giemsa staining of sister chromatids and the study of chromatid exchanges without autoradiography.

Authors:  S Wolff; P Perry
Journal:  Chromosoma       Date:  1974       Impact factor: 4.316

8.  Possible role of DNA synthesis in formation of sister chromatid exchanges.

Authors:  H Kato
Journal:  Nature       Date:  1974-12-20       Impact factor: 49.962

9.  DNA replication sequence of human chromosomes in blood cultures.

Authors:  N O Bianchi; M S De Bianchi
Journal:  Chromosoma       Date:  1965       Impact factor: 4.316
  9 in total
  2 in total

1.  Kinetics of division in PHA-stimulated pig lymphocytes.

Authors:  E A Lezana; M S Bianchi; N O Bianchi
Journal:  Experientia       Date:  1978-01-15

2.  Human lymphocyte cell cycle: studies with the use of BrUdR.

Authors:  P Auf der Maur; K Berlincourt-Böhni
Journal:  Hum Genet       Date:  1979-06-19       Impact factor: 4.132
  2 in total

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