PROBLEM: The low frequency of maternal immune responses to paternally inherited fetal antigens raises the following question: What regulates the immunobiology of pregnancy? Data suggest that this state is the result of peripheral immune-tolerance, an active process of immune-regulation in which activated T cells undergo apoptosis. We studied Fas ligand (FasL) expression and apoptosis in normal and pathologic placentas to find out whether the Fas-FasL-induced apoptosis takes place during implantation. METHOD OF STUDY: FasL expression in paraffin sections was detected using specific antibodies and confirmed with reverse transcriptase-polymerase chain reaction of total RNA from frozen placentas. Apoptosis was detected using the terminal deoxy (d)-UTP nick end-labeling assay. RESULTS: FasL was found in the normal placenta and in gestational trophoblastic disease. Apoptotic leukocytes were localized to the maternal-fetal interface corresponding in localization with the distribution of FasL. CONCLUSIONS: We propose that FasL expression in the placenta is a mechanism responsible for the development of maternal immune tolerance specific for paternal alloantigens and operates in pathologic states characterized by trophoblastic invasion/proliferation.
PROBLEM: The low frequency of maternal immune responses to paternally inherited fetal antigens raises the following question: What regulates the immunobiology of pregnancy? Data suggest that this state is the result of peripheral immune-tolerance, an active process of immune-regulation in which activated T cells undergo apoptosis. We studied Fas ligand (FasL) expression and apoptosis in normal and pathologic placentas to find out whether the Fas-FasL-induced apoptosis takes place during implantation. METHOD OF STUDY: FasL expression in paraffin sections was detected using specific antibodies and confirmed with reverse transcriptase-polymerase chain reaction of total RNA from frozen placentas. Apoptosis was detected using the terminal deoxy(d)-UTP nick end-labeling assay. RESULTS:FasL was found in the normal placenta and in gestational trophoblastic disease. Apoptotic leukocytes were localized to the maternal-fetal interface corresponding in localization with the distribution of FasL. CONCLUSIONS: We propose that FasL expression in the placenta is a mechanism responsible for the development of maternal immune tolerance specific for paternal alloantigens and operates in pathologic states characterized by trophoblastic invasion/proliferation.
Authors: V Yu Talayev; A V Matveichev; M A Lomunova; M V Talayeva; M E Tsaturov; I Ye Zaichenko; O N Babaykina Journal: Clin Exp Immunol Date: 2010-08-19 Impact factor: 4.330
Authors: Jacob Gibbens; Shauna-Kay Spencer; Lucia Solis; Teylor Bowles; Patrick B Kyle; Jamie L Szczepanski; John Polk Dumas; Reanna Robinson; Kedra Wallace Journal: Am J Physiol Regul Integr Comp Physiol Date: 2020-07-08 Impact factor: 3.619
Authors: J Song; E Sapi; W Brown; J Nilsen; K Tartaro; B M Kacinski; J Craft; F Naftolin; G Mor Journal: J Clin Invest Date: 2000-11 Impact factor: 14.808
Authors: Lynda K Harris; Rosemary J Keogh; Mark Wareing; Philip N Baker; Judith E Cartwright; John D Aplin; Guy St J Whitley Journal: Am J Pathol Date: 2006-11 Impact factor: 4.307
Authors: Tinnakorn Chaiworapongsa; Roberto Romero; Francesca Gotsch; Jimmy Espinoza; Jyh Kae Nien; Luis Goncalves; Samuel Edwin; Yeon Mee Kim; Offer Erez; Juan Pedro Kusanovic; Beth L Pineles; Zoltan Papp; Sonia Hassan Journal: J Matern Fetal Neonatal Med Date: 2008-01