Literature DB >> 9762147

A simple method for synthesizing [gamma-32P]nucleoside triphosphates using [32P]acetylphosphate and acetate kinase.

P I Bauer1, G Várady.   

Abstract

A simple, rapid, and inexpensive method is described for the synthesis of gamma-32P-labeled ribo- or deoxyribonucleoside triphosphates. The procedure involves chemical synthesis of [32P]acetylphosphate and subsequent phosphorylation of nucleoside diphosphates using acetate kinase (EC 2.7.2.1) and a final purification step. The entire procedure is performed 8 h or less.

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Year:  1978        PMID： 9762147     DOI： 10.1016/0003-2697(78)90547-x

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  2 in total

1.  Preparation of a mixture of nucleoside triphosphates suitable for use in synthesis of nucleotide phosphate sugars from ribonucleic acid using nuclease P1, a mixture of nucleoside monophosphokinases and acetate kinase.

Authors:  S L Haynie; G M Whitesides
Journal:  Appl Biochem Biotechnol       Date:  1990-03       Impact factor: 2.926

2.  Long-term effect of cyclic AMP on N-glycosylation is caused by an increase in the activity of the cis-prenyltransferase.

Authors:  M Konrad; W E Merz
Journal:  Biochem J       Date:  1996-06-01       Impact factor: 3.857
  2 in total

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