Literature DB >> 9761926

Expression, purification, crystallization and crystallographic characterization of the human MHC class I related protein MICA.

S Bauer1, S T Willie, T Spies, R K Strong.   

Abstract

Crystals of the human MHC-encoded molecule MICA, a homologue of MHC class I proteins, have been grown in hanging-drop vapor-diffusion trials using ammonium sulfate as a precipitating agent with recombinant protein expressed in a baculovirus-based system. Cryo-preserved crystals of MICA belong to the cubic space group F4132 with lattice constants a = b = c = 260.7 A and diffract to a resolution limit of 3.0 A when cryo-preserved. These crystals do not diffract when handled conventionally.

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Year:  1998        PMID： 9761926     DOI： 10.1107/s0907444997015229

Source DB:  PubMed          Journal:  Acta Crystallogr D Biol Crystallogr        ISSN： 0907-4449


  5 in total

1.  Diversification, expression, and gamma delta T cell recognition of evolutionarily distant members of the MIC family of major histocompatibility complex class I-related molecules.

Authors:  A Steinle; V Groh; T Spies
Journal:  Proc Natl Acad Sci U S A       Date:  1998-10-13       Impact factor: 11.205

2.  Prevalent expression of the immunostimulatory MHC class I chain-related molecule is counteracted by shedding in prostate cancer.

Authors:  Jennifer D Wu; Lily M Higgins; Alexander Steinle; David Cosman; Kathy Haugk; Stephen R Plymate
Journal:  J Clin Invest       Date:  2004-08       Impact factor: 14.808

Review 3.  Murine NKG2D ligands: "double, double toil and trouble".

Authors:  Asanga Samarakoon; Haiyan Chu; Subramaniam Malarkannan
Journal:  Mol Immunol       Date:  2008-12-10       Impact factor: 4.407

4.  Loss of expression of MHC class I-related chain A (MICA) is a frequent event and predicts poor survival in patients with hepatocellular carcinoma.

Authors:  Jie Zhang; Zhuding Xu; Xingchun Zhou; Haibin Zhang; Ning Yang; Yousheng Wu; Yibing Chen; Guangshun Yang; Tingting Ren
Journal:  Int J Clin Exp Pathol       Date:  2014-05-15

5.  Daedalus: a robust, turnkey platform for rapid production of decigram quantities of active recombinant proteins in human cell lines using novel lentiviral vectors.

Authors:  Ashok D Bandaranayake; Colin Correnti; Byoung Y Ryu; Michelle Brault; Roland K Strong; David J Rawlings
Journal:  Nucleic Acids Res       Date:  2011-09-12       Impact factor: 16.971
  5 in total

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