PCR-based methods for identifying genetic variations in human alpha1B- and beta2-adrenergic receptors.

alpha- and beta-adrenergic receptors belong to the superfamily of G-protein-coupled, seven transmembrane domain receptors and regulate a variety of cellular processes. Previous studies have demonstrated that changes in the amino acid sequence can result in substantial changes in the function of the receptors and it has been suggested that there may be an association between different disease states and the altered structure of alpha- and beta-adrenergic receptors. Accordingly, we have developed a simple PCR method for the identification of polymorphisms in the coding sequences of the human beta2-adrenergic receptor and the alpha1B-adrenergic receptor. This method may be useful for screening individual patients or at-risk populations for endocrine-metabolic disorders, as well as for asthma, cardiovascular disorders, and neuropsychiatric diseases. Copyright 1998 Academic Press.