PURPOSE: This study evaluated the ability of partial liquid ventilation (PLV, gas ventilation of the perfluorocarbon-filled lungs) to reduce the amount of lung albumin leak present in the setting of acute lung injury. MATERIALS AND METHODS: An experimental controlled, randomized design was used. All studies were performed in the liquid ventilation laboratories at the University of Michigan Medical Center. Twenty-five Sprague-Dawley male rats 500+/-50 g were divided into five experimental groups: (1) CVF only (n=5), animals were cobra venom factor (CVF) lung injured; (2) PLV-CVF (n=5) animals received perflubron and PLV before CVF lung injury; (3) CVF-PLV (n=5) animals received PLV after CVF lung injury; (4) PLV only (n=5) animals underwent partial liquid ventilation without lung injury; and (5) Gas only (n=5) animals underwent gas ventilation without lung injury. In all groups iodinated bovine serum albumin (125I-BSA) was delivered by intravenous injection along with CVF or a saline placebo. RESULTS: When the CVF animals were compared with all other groups, a decrease in albumin leak was observed for all groups when compared with the CVF only controls (P < .001 by ANOVA; CVF only=1.22+/-0.12 versus PLV-CVF=0.46+/-0.08, P < .001; CVF-PLV=0.70+/-0.25, P < .001; PLV only=0.22+/-0.01, P < .001; Gas only=0.17+/-0.02, P < .001). CONCLUSIONS: These data suggest that intratracheal instillation of perfluorocarbon before or after induction of lung injury results in a reduction in pulmonary albumin leak.
PURPOSE: This study evaluated the ability of partial liquid ventilation (PLV, gas ventilation of the perfluorocarbon-filled lungs) to reduce the amount of lung albumin leak present in the setting of acute lung injury. MATERIALS AND METHODS: An experimental controlled, randomized design was used. All studies were performed in the liquid ventilation laboratories at the University of Michigan Medical Center. Twenty-five Sprague-Dawley male rats 500+/-50 g were divided into five experimental groups: (1) CVF only (n=5), animals were cobra venom factor (CVF) lung injured; (2) PLV-CVF (n=5) animals received perflubron and PLV before CVF lung injury; (3) CVF-PLV (n=5) animals received PLV after CVF lung injury; (4) PLV only (n=5) animals underwent partial liquid ventilation without lung injury; and (5) Gas only (n=5) animals underwent gas ventilation without lung injury. In all groups iodinated bovine serum albumin (125I-BSA) was delivered by intravenous injection along with CVF or a saline placebo. RESULTS: When the CVF animals were compared with all other groups, a decrease in albumin leak was observed for all groups when compared with the CVF only controls (P < .001 by ANOVA; CVF only=1.22+/-0.12 versus PLV-CVF=0.46+/-0.08, P < .001; CVF-PLV=0.70+/-0.25, P < .001; PLV only=0.22+/-0.01, P < .001; Gas only=0.17+/-0.02, P < .001). CONCLUSIONS: These data suggest that intratracheal instillation of perfluorocarbon before or after induction of lung injury results in a reduction in pulmonary albumin leak.
Authors: Angela C Ko; Emily Hirsh; Andrew C Wong; Timothy M Moore; Aubrey E Taylor; Ronald B Hirschl; John G Younger Journal: Resuscitation Date: 2003-04 Impact factor: 5.262