OBJECTIVE: To determine the presence of FSH in unextracted urine of perimenopausal women using amicroparticle enzyme immunoassay kit on an AxSYM random access immunoassay analyzer. DESIGN: Controlled descriptive study. SETTING: A large teaching hospital and infertility clinic. PATIENT(S): Forty perimenopausal women aged 32-55 years admitted to our clinic for a gynecological operation. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Mean serum FSH level and urinary FSH in early-morning urine, in a random void urine sample, and in 24-hour urine on the same day. FSH in urine on the day of excretion and 1 and 4 weeks thereafter, stored under various conditions. FSH in urine before and after extraction. RESULT(S): The Pearson's correlation coefficient between mean serum FSH levels and urinary FSH in early morning urine was 0.904, in a random void 0.915, and in 24-hour urine 0.857. Determination of optimal storage conditions revealed that urine was best kept at 4 degrees C without any additive. The correlation between FSH in extracted and unextracted urine was 98.9%. CONCLUSION(S): In perimenopausal women, FSH can be reliably measured in unextracted urine. The correlation between urinary FSH and a random void urine sample and mean FSH from a serial serum sample is very high. Urine can be stored for 4 weeks at 4 degrees C without loss of FSH immunoreactivity.
RCT Entities:
OBJECTIVE: To determine the presence of FSH in unextracted urine of perimenopausal women using a microparticle enzyme immunoassay kit on an AxSYM random access immunoassay analyzer. DESIGN: Controlled descriptive study. SETTING: A large teaching hospital and infertility clinic. PATIENT(S): Forty perimenopausal women aged 32-55 years admitted to our clinic for a gynecological operation. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Mean serum FSH level and urinary FSH in early-morning urine, in a random void urine sample, and in 24-hour urine on the same day. FSH in urine on the day of excretion and 1 and 4 weeks thereafter, stored under various conditions. FSH in urine before and after extraction. RESULT(S): The Pearson's correlation coefficient between mean serum FSH levels and urinary FSH in early morning urine was 0.904, in a random void 0.915, and in 24-hour urine 0.857. Determination of optimal storage conditions revealed that urine was best kept at 4 degrees C without any additive. The correlation between FSH in extracted and unextracted urine was 98.9%. CONCLUSION(S): In perimenopausal women, FSH can be reliably measured in unextracted urine. The correlation between urinary FSH and a random void urine sample and mean FSH from a serial serum sample is very high. Urine can be stored for 4 weeks at 4 degrees C without loss of FSH immunoreactivity.
Authors: Anne Z Steiner; D Leann Long; Amy H Herring; James S Kesner; Juliana W Meadows; Donna D Baird Journal: Reprod Sci Date: 2012-11-20 Impact factor: 3.060
Authors: Jessica Scriver; Valerie L Baker; Steven L Young; Barry Behr; Lisa M Pastore Journal: Reprod Biol Endocrinol Date: 2010-11-29 Impact factor: 5.211