Literature DB >> 9751802

A new set of positive/negative selectable markers for mammalian cells.

C Karreman1.   

Abstract

Five new positive and negative selectable markers were created for use in mammalian cells. Their negative selectabilities are based on the Thymidine kinase (Tk) gene of Herpes Simplex virus (HSV) or the Cytidine deaminase (codA) gene of E. coli. The markers can be selected positively by their ability to induce either Hygromycin (Hyg), neomycin (neo), puromycin (PAC) or Blasticidin S (BlaS) resistance. With these markers, two complete sets of markergenes are available that induce independent negative selectable phenotypes.

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Year:  1998        PMID： 9751802     DOI： 10.1016/s0378-1119(98)00387-4

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  9 in total

1.  Genetic selection scheme for isolation of signal transduction pathway mutants.

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Journal:  Appl Environ Microbiol       Date:  2004-02       Impact factor: 4.792

2.  Retention of the developmental pluripotency in medaka embryonic stem cells after gene transfer and long-term drug selection for gene targeting in fish.

Authors:  Yunhan Hong; Songlin Chen; Jianfang Gui; Manfred Schartl
Journal:  Transgenic Res       Date:  2004-02       Impact factor: 2.788

3.  Genetic interactions between hepatitis C virus replicons.

Authors:  Matthew J Evans; Charles M Rice; Stephen P Goff
Journal:  J Virol       Date:  2004-11       Impact factor: 5.103

4.  Application of counter-selectable marker PIGA in engineering designer deletion cell lines and characterization of CRISPR deletion efficiency.

Authors:  Donghui Li; Xiaoji Sun; Fangzhou Yu; Mary Ann Perle; David Araten; Jef D Boeke
Journal:  Nucleic Acids Res       Date:  2021-03-18       Impact factor: 16.971

5.  Development and application of a positive-negative selectable marker system for use in reverse genetics in Plasmodium.

Authors:  Joanna A M Braks; Blandine Franke-Fayard; Hans Kroeze; Chris J Janse; Andrew P Waters
Journal:  Nucleic Acids Res       Date:  2006-03-14       Impact factor: 16.971

Review 6.  Pigs taking wing with transposons and recombinases.

Authors:  Karl J Clark; Daniel F Carlson; Scott C Fahrenkrug
Journal:  Genome Biol       Date:  2007       Impact factor: 13.583

7.  An efficient system to establish multiple embryonic stem cell lines carrying an inducible expression unit.

Authors:  Shinji Masui; Daisuke Shimosato; Yayoi Toyooka; Rika Yagi; Kazue Takahashi; Hitoshi Niwa
Journal:  Nucleic Acids Res       Date:  2005-03-01       Impact factor: 16.971

8.  Homology Requirements for Efficient, Footprintless Gene Editing at the CFTR Locus in Human iPSCs with Helper-dependent Adenoviral Vectors.

Authors:  Donna J Palmer; Nathan C Grove; Jordan Ing; Ana M Crane; Koen Venken; Brian R Davis; Philip Ng
Journal:  Mol Ther Nucleic Acids       Date:  2016-10-11       Impact factor: 10.183

9.  Bi-allelic Homology-Directed Repair with Helper-Dependent Adenoviruses.

Authors:  Donna J Palmer; Dustin L Turner; Philip Ng
Journal:  Mol Ther Methods Clin Dev       Date:  2019-10-17       Impact factor: 6.698
  9 in total

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