Literature DB >> 9750326

Escherichia coli O157 serology: false-positive ELISA results caused by human antibodies binding to bovine serum albumin.

H Chart1, J Evans, R M Chalmers, R L Salmon.   

Abstract

An analysis of farm workers and rural dwellers for serum antibodies to the lipopolysaccharide (LPS) of Escherichia coli O157 detected sera with antibodies binding to bovine serum albumin (BSA) by ELISA. These antibodies were not specific for BSA when examined by immunoblotting, and the ELISA values were reduced to a background level when plates were blocked with normal rabbit serum.

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Year:  1998        PMID： 9750326     DOI： 10.1046/j.1472-765x.1998.00396.x

Source DB:  PubMed          Journal:  Lett Appl Microbiol        ISSN： 0266-8254            Impact factor:   2.858


  3 in total

1.  Evidence of persisting serum antibodies to Escherichia coli O157 lipopolysaccharide and Verocytotoxin in members of rural communities in England.

Authors:  J Evans; R M Chalmers; H Chart; R L Salmon; S M Kench; T J Coleman; D Meadows; P Morgan-Capner; P Softley; M Sillis; D R Thomas
Journal:  Eur J Epidemiol       Date:  2000       Impact factor: 8.082

2.  Enzyme-linked immunosorbent assay (ELISA) and blocking with bovine serum albumin (BSA)--not all BSAs are alike.

Authors:  Yuhong Xiao; Stuart N Isaacs
Journal:  J Immunol Methods       Date:  2012-06-23       Impact factor: 2.303

3.  Evaluation of Hydra HALT-1 as a toxin moiety for recombinant immunotoxin.

Authors:  William F Jiemy; Lih Fhung Hiew; Hong Xi Sha; Lionel L A In; Jung Shan Hwang
Journal:  BMC Biotechnol       Date:  2020-06-17       Impact factor: 2.563
  3 in total

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