O A Khan1, S S Dhib-Jalbut. 1. Department of Neurology, University of Maryland School of Medicine, Veterans Affairs Medical Center, Baltimore, USA.
Abstract
BACKGROUND: The pharmacokinetics of IFNbeta-1a in MS patients are poorly understood. We have previously reported an ELISA sensitive and specific for measuring serum IFNbeta-1b levels in patients with MS. OBJECTIVE: We describe an ELISA to measure interferon beta-1a (Avonex) in the serum of MS patients following IM administration. METHODS: We have developed an ELISA for detecting serum IFNbeta-1a in MS patients receiving 6 million units (MU) of IFNbeta-1a, IM once weekly. The specificity of this ELISA was confirmed by the lack of cross-reactivity with other cytokines except for IFNbeta-1b. RESULTS: Serum IFNbeta-1a levels were measured at 3 and 6 months after initiating treatment with IFNbeta-1a in 10 MS patients. At 3 months, all 10 patients had detectable levels ranging from 68 to 86 IU/mL. At 6 months, IFNbeta-1a could be detected in the serum of all but three patients, with levels ranging from 64 to 81 IU/mL. A kinetic study of IFNbeta-1a serum levels in a separate group of six MS patients who had been receiving IFNbeta-1a for several months was carried out. Blood was drawn before and 2, 4, 6, 8, and 24 hours after IFNbeta-1a injection. Peak serum IFNbeta-1a levels were observed at 8 hours and became undetectable at 24 hours after injection. CONCLUSION: The described ELISA may have useful clinical applications in examining the correlation between serum IFNbeta-1a levels and clinical efficacy.
BACKGROUND: The pharmacokinetics of IFNbeta-1a in MSpatients are poorly understood. We have previously reported an ELISA sensitive and specific for measuring serum IFNbeta-1b levels in patients with MS. OBJECTIVE: We describe an ELISA to measure interferon beta-1a (Avonex) in the serum of MSpatients following IM administration. METHODS: We have developed an ELISA for detecting serum IFNbeta-1a in MSpatients receiving 6 million units (MU) of IFNbeta-1a, IM once weekly. The specificity of this ELISA was confirmed by the lack of cross-reactivity with other cytokines except for IFNbeta-1b. RESULTS: Serum IFNbeta-1a levels were measured at 3 and 6 months after initiating treatment with IFNbeta-1a in 10 MSpatients. At 3 months, all 10 patients had detectable levels ranging from 68 to 86 IU/mL. At 6 months, IFNbeta-1a could be detected in the serum of all but three patients, with levels ranging from 64 to 81 IU/mL. A kinetic study of IFNbeta-1a serum levels in a separate group of six MSpatients who had been receiving IFNbeta-1a for several months was carried out. Blood was drawn before and 2, 4, 6, 8, and 24 hours after IFNbeta-1a injection. Peak serum IFNbeta-1a levels were observed at 8 hours and became undetectable at 24 hours after injection. CONCLUSION: The described ELISA may have useful clinical applications in examining the correlation between serum IFNbeta-1a levels and clinical efficacy.
Authors: Joana A Zula; Holly C Green; Richard M Ransohoff; Richard A Rudick; George R Stark; Anette H H van Boxel-Dezaire Journal: Proc Natl Acad Sci U S A Date: 2011-11-21 Impact factor: 11.205
Authors: Alexey S Kazakov; Alexander D Sofin; Nadezhda V Avkhacheva; Alexander I Denesyuk; Evgenia I Deryusheva; Victoria A Rastrygina; Andrey S Sokolov; Maria E Permyakova; Ekaterina A Litus; Vladimir N Uversky; Eugene A Permyakov; Sergei E Permyakov Journal: Int J Mol Sci Date: 2020-12-13 Impact factor: 5.923
Authors: Alexey S Kazakov; Alexander D Sofin; Nadezhda V Avkhacheva; Evgenia I Deryusheva; Victoria A Rastrygina; Maria E Permyakova; Vladimir N Uversky; Eugene A Permyakov; Sergei E Permyakov Journal: Int J Mol Sci Date: 2022-02-11 Impact factor: 5.923