| Literature DB >> 9740050 |
M Chevallet1, V Santoni, A Poinas, D Rouquié, A Fuchs, S Kieffer, M Rossignol, J Lunardi, J Garin, T Rabilloud.
Abstract
Severe quantitative loss of protein is often observed in high-resolution two-dimensional electrophoresis of membrane proteins, while the resolution is usually not affected. To improve the solubility of proteins in this technique, we tested denaturing cocktails containing various detergents and chaotropes. Best results were obtained with a denaturing solution containing urea, thiourea, and zwitterionic detergents, synthesized for this purpose. Among the dozen detergents synthesized and tested, amidosulfobetaines with an alkyl tail containing 14-16 carbons proved most efficient, solubilizing previously undetected membrane proteins.Entities:
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Year: 1998 PMID： 9740050 DOI： 10.1002/elps.1150191108
Source DB: PubMed Journal: Electrophoresis ISSN： 0173-0835 Impact factor: 3.535