Literature DB >> 974

Separation of deoxyribonucleases (DNases) of normal human stratum corneum and psoriatic scales by micro-disc-electrophoresis.

F J Förster, G Leonhardi, E J Zöllner, R K Zahn.   

Abstract

Normal stratum corneum and psoriatic scales were homogenized and a differential centrifugation was performed. The DNase activity of the individual fractions was investigated by micro-dis-electrophoresis. At pH 5 only in the 600xg pellet and 105.000xg supernatant of normal keratin DNase activity could be observed. However, all psoriatic fractions showed distinct enzyme activities. At pH 7.4 little psoriatic DNase activity could only be demonstrated in the 105.000xg supernatant. Except from the 15.000xg pellet all fractions of normal stratum corneum displayed marked activities. In addition the 105.000xg supernatant showed two different DNase bands.

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Year:  1975        PMID： 974     DOI： 10.1007/BF00561146

Source DB:  PubMed          Journal:  Arch Dermatol Res            Impact factor:   3.017


  9 in total

1.  Subcellular distribution of phosphatases, proteinases, and ribonucleases in normal human stratum corneum and psoriatic scales.

Authors:  F J Förster; A Neufahrt; G Stockum; K Bauer; S Frenkel; U Fertig; G Leonhardi
Journal:  Arch Dermatol Res       Date:  1975-11-14       Impact factor: 3.017

2.  [ON THE LOCALIZATION OF RIBONUCLEASE (RNASE) AND DESOXYRIBONUCLEASE (DNASE) ACTIVITY IN NORMAL AND INFLAMED SKIN AND IN SKIN TUMORS].

Authors:  G K STEIGLEDER; I FISCHER
Journal:  Arch Klin Exp Dermatol       Date:  1963-12-10

3.  [BASIC BIOCHEMICAL PROBLEMS IN EPIDERMAL KERATINIZATION].

Authors:  H W SPIER
Journal:  Arch Klin Exp Dermatol       Date:  1964-06-25

4.  [A preservation and preparation process for desoxyribonucleic acids and their raw materials].

Authors:  R K ZAHN; E TIESLER; A K KLEINSCHMIDT; D LANG
Journal:  Biochem Z       Date:  1962

5.  The localization of ribonuclease and deoxyribonuclease activities in normal and psoriatic epidermis.

Authors:  G K STEIGLEDER; W P RAAB
Journal:  J Invest Dermatol       Date:  1962-04       Impact factor: 8.551

6.  [Histochemistry of hyperkeratosis].

Authors:  H W SPIER; P VAN CANEGHEM
Journal:  Arch Klin Exp Dermatol       Date:  1957

7.  [Serum deoxyribonucleases and DNA-antibodies in lupus erythematodes (author's transl)].

Authors:  E J Zöllner; B Heicke; R K Zahn
Journal:  Z Klin Chem Klin Biochem       Date:  1974-10

8.  [Separation of psoriatic scale proteinases by differential centrifugation and gel filtration (author's transl)].

Authors:  A Neufahrt; S Henckel
Journal:  Arch Dermatol Forsch       Date:  1974

9.  [Detection of several different nuclease activities in human cerebrospinal fluid (author's transl)].

Authors:  E J Zöllner; R K Zahn
Journal:  Z Klin Chem Klin Biochem       Date:  1973-03
  9 in total
  7 in total

1.  Isolation and physicochemical characterisation of the DNA from normal human skin and psoriatic scales.

Authors:  G Teimer; G Ramb; G Leonhardi
Journal:  Arch Dermatol Res       Date:  1976-10-27       Impact factor: 3.017

2.  Microdisc-electrophoretic study of deoxyribonucleases in cow snout epidermis.

Authors:  R Ogura; T Ueda; S Kumano; T Sakata; E J Zöllner; R K Zahn
Journal:  Arch Dermatol Res       Date:  1983       Impact factor: 3.017

Review 3.  Microelectrophoresis as a tool in enzyme histochemistry.

Authors:  G Huether; V Neuhoff
Journal:  Histochem J       Date:  1981-03

4.  Disc-electrophoretic separation of water soluble proteins from scales of different forms of ichthyosis.

Authors:  G Leonhardi; L Ross; A Neufahrt
Journal:  Arch Dermatol Res       Date:  1977-12-27       Impact factor: 3.017

5.  Comparison of DNase, DNA-polymerase and RNA-polymerase activities present in the DNA-binding proteins of normal human dermis, epidermis, horny layer and psoriatic scales.

Authors:  G Reimer; E J Zöllner; M Reitz; U Schwulera; G Leonhardi
Journal:  Arch Dermatol Res       Date:  1978-12-01       Impact factor: 3.017

6.  DNase 2 is the main DNA-degrading enzyme of the stratum corneum.

Authors:  Heinz Fischer; Jennifer Scherz; Sandra Szabo; Michael Mildner; Charaf Benarafa; Alicia Torriglia; Erwin Tschachler; Leopold Eckhart
Journal:  PLoS One       Date:  2011-03-01       Impact factor: 3.240

7.  Inactivation of DNase1L2 and DNase2 in keratinocytes suppresses DNA degradation during epidermal cornification and results in constitutive parakeratosis.

Authors:  Heinz Fischer; Maria Buchberger; Markus Napirei; Erwin Tschachler; Leopold Eckhart
Journal:  Sci Rep       Date:  2017-07-25       Impact factor: 4.379
  7 in total

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