| Literature DB >> 973935 |
B L Hillcoat, M Kawai, P B McCulloch, J Rosenfeld, C K Williams.
Abstract
1 A gas chromatographic-mass spectrometric method was developed for determining 5-fluorouracil in plasma, using methylated thymine as an internal standard. 2 5-fluorouracil was extracted from plasma by a novel procedure which removed plasma components interferring with the sensitivity of the assay. The method included heating the plasma, washing with ether and extracting the drug under optimum conditions. 3 The sensitivity of the assay was 10 ng/ml plasma, sufficient to determine the low concentrations of 5-fluorouracil found in plasma during continuous infusion of the drug in patients receiving chemotherapy for cancer.Entities:
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Year: 1976 PMID： 973935 PMCID： PMC1428807 DOI： 10.1111/j.1365-2125.1976.tb00580.x
Source DB: PubMed Journal: Br J Clin Pharmacol ISSN： 0306-5251 Impact factor: 4.335