P R Ling1, P Boyce, B R Bistrian. 1. Nutrition/Infection Laboratory, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA.
Abstract
BACKGROUND: This study examined whether adding arachidonic acid (AA) to a fish oil diet would alter certain of the anti-inflammatory effects of fish oil in response to tumor necrosis factor (TNF) infusion in rats. METHODS: AA was given at 0.08 wt% of diet for 6 weeks. The total fat in each diet provided 20% of dietary energy. Four groups were pair-fed sunflower oil (S), S+AA, fish oil (F), or F+AA for 6 weeks. At the end of feeding, each animal received TNF-alpha (20 microg/kg) infusion for 3 hours. After 1 hour of TNF infusion, a euglycemic and hyperinsulinemic clamp (10 mU/min per kilogram of insulin) was used to determine the actions of insulin. The insulin-stimulated glucose utilization in liver, muscle, and fat was determined by using 14C-deoxyglucose. The plasma glucose, insulin, and corticosterone levels were determined at basal, 60 minutes, and the end of the experiment (180 minutes). The fatty acid composition of plasma phospholipids also was determined. RESULTS: Fish oil significantly increased omega-3 fatty acids in phospholipids in both F and F+AA and decreased AA in F, compared with S. AA significantly restored the level of AA and reduced the increase of omega-3 fatty acids in phospholipids in F+AA compared with F, but had no impact on fatty acid composition when added to S. Corticosterone level was significantly lower with fish oil feeding but higher in both F and S containing AA compared with F and S, respectively. The highest glucose uptake in tissues was in F, followed by F+AA, and then S and S+AA. CONCLUSIONS: These results suggest that fish oil is anti-inflammatory principally through a reduction in the AA content of phospholipids.
BACKGROUND: This study examined whether adding arachidonic acid (AA) to a fish oil diet would alter certain of the anti-inflammatory effects of fish oil in response to tumor necrosis factor (TNF) infusion in rats. METHODS: AA was given at 0.08 wt% of diet for 6 weeks. The total fat in each diet provided 20% of dietary energy. Four groups were pair-fed sunflower oil (S), S+AA, fish oil (F), or F+AA for 6 weeks. At the end of feeding, each animal received TNF-alpha (20 microg/kg) infusion for 3 hours. After 1 hour of TNF infusion, a euglycemic and hyperinsulinemic clamp (10 mU/min per kilogram of insulin) was used to determine the actions of insulin. The insulin-stimulated glucose utilization in liver, muscle, and fat was determined by using 14C-deoxyglucose. The plasma glucose, insulin, and corticosterone levels were determined at basal, 60 minutes, and the end of the experiment (180 minutes). The fatty acid composition of plasma phospholipids also was determined. RESULTS: Fish oil significantly increased omega-3 fatty acids in phospholipids in both F and F+AA and decreased AA in F, compared with S. AA significantly restored the level of AA and reduced the increase of omega-3 fatty acids in phospholipids in F+AA compared with F, but had no impact on fatty acid composition when added to S. Corticosterone level was significantly lower with fish oil feeding but higher in both F and S containing AA compared with F and S, respectively. The highest glucose uptake in tissues was in F, followed by F+AA, and then S and S+AA. CONCLUSIONS: These results suggest that fish oil is anti-inflammatory principally through a reduction in the AA content of phospholipids.
Authors: Hau D Le; Jonathan A Meisel; Vincent E de Meijer; Erica M Fallon; Kathleen M Gura; Vania Nose; Bruce R Bistrian; Mark Puder Journal: JPEN J Parenter Enteral Nutr Date: 2011-10-30 Impact factor: 4.016
Authors: Sarah J Carlson; Prathima Nandivada; Melissa I Chang; Paul D Mitchell; Alison O'Loughlin; Eileen Cowan; Kathleen M Gura; Vania Nose; Bruce R Bistrian; Mark Puder Journal: Metabolism Date: 2014-10-13 Impact factor: 8.694
Authors: Hau D Le; Erica M Fallon; Brian T Kalish; Vincent E de Meijer; Jonathan A Meisel; Kathleen M Gura; Vania Nose; Amy H Pan; Bruce R Bistrian; Mark Puder Journal: Metabolism Date: 2012-11-12 Impact factor: 8.694
Authors: Margo N Woods; Christine A Wanke; Pei-Ra Ling; Kristy M Hendricks; Alice M Tang; Tamsin A Knox; Charlotte E Andersson; Kimberly R Dong; Sally C Skinner; Bruce R Bistrian Journal: Am J Clin Nutr Date: 2009-10-21 Impact factor: 7.045
Authors: Pei-Ra Ling; Mario Ollero; Lalita Khaodhiar; Karen McCowen; Mary Keane-Ellison; Ann Thibault; Nicholas Tawa; Bruce R Bistrian Journal: Dig Dis Sci Date: 2002-08 Impact factor: 3.199
Authors: Michelle S Dotzert; Michael R Murray; Matthew W McDonald; T Dylan Olver; Thomas J Velenosi; Anzel Hennop; Earl G Noble; Brad L Urquhart; C W James Melling Journal: Sci Rep Date: 2016-05-20 Impact factor: 4.379