Literature DB >> 9733835

trans-encapsidation of a poliovirus replicon by different picornavirus capsid proteins.

X Y Jia1, M Van Eden, M G Busch, E Ehrenfeld, D F Summers.   

Abstract

A trans-encapsidation assay was established to study the specificity of picornavirus RNA encapsidation. A poliovirus replicon with the luciferase gene replacing the capsid protein-coding region was coexpressed in transfected HeLa cells with capsid proteins from homologous or heterologous virus. Successful trans-encapsidation resulted in assembly and production of virions whose replication, upon subsequent infection of HeLa cells, was accompanied by expression of luciferase activity. The amount of luciferase activity was proportional to the amount of trans-encapsidated virus produced from the cotransfection. When poliovirus capsid proteins were supplied in trans, >2 x 10(6) infectious particles/ml were produced. When coxsackievirus B3, human rhinovirus 14, mengovirus, or hepatitis A virus (HAV) capsid proteins were supplied in trans, all but HAV showed some encapsidation of the replicon. The overall encapsidation efficiency of the replicon RNA by heterologous capsid proteins was significantly lower than when poliovirus capsid was used. trans-encapsidated particles could be completely neutralized with specific antisera against each of the donor virus capsids. The results indicate that encapsidation is regulated by specific viral nucleic acid and protein sequences.

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Mesh：

Year:  1998        PMID： 9733835      PMCID： PMC110132     

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  34 in total

1.  Atomic structure of single-stranded DNA bacteriophage phi X174 and its functional implications.

Authors:  R McKenna; D Xia; P Willingmann; L L Ilag; S Krishnaswamy; M G Rossmann; N H Olson; T S Baker; N L Incardona
Journal:  Nature       Date:  1992-01-09       Impact factor: 49.962

2.  MATURATION OF POLIOVIRUS RNA WITH CAPSID PROTEIN CODED BY HETEROLOGOUS ENTEROVIRUSES.

Authors:  J J HOLLAND; C E CORDS
Journal:  Proc Natl Acad Sci U S A       Date:  1964-06       Impact factor: 11.205

3.  Characterization of a new isolate of poliovirus defective interfering particles.

Authors:  R E Lundquist; M Sullivan; J V Maizel
Journal:  Cell       Date:  1979-11       Impact factor: 41.582

4.  A genome-linked copy of the NS-1 polypeptide is located on the outside of infectious parvovirus particles.

Authors:  S F Cotmore; P Tattersall
Journal:  J Virol       Date:  1989-09       Impact factor: 5.103

5.  Structure and assembly of turnip crinkle virus. VI. Identification of coat protein binding sites on the RNA.

Authors:  N Wei; L A Heaton; T J Morris; S C Harrison
Journal:  J Mol Biol       Date:  1990-07-05       Impact factor: 5.469

6.  A cellular protein that binds to the 5'-noncoding region of poliovirus RNA: implications for internal translation initiation.

Authors:  K Meerovitch; J Pelletier; N Sonenberg
Journal:  Genes Dev       Date:  1989-07       Impact factor: 11.361

Review 7.  DNA packaging in dsDNA bacteriophages.

Authors:  L W Black
Journal:  Annu Rev Microbiol       Date:  1989       Impact factor: 15.500

8.  Cloning and synthesis of infectious cardiovirus RNAs containing short, discrete poly(C) tracts.

Authors:  G M Duke; A C Palmenberg
Journal:  J Virol       Date:  1989-04       Impact factor: 5.103

9.  The 5'-terminal nucleotides of hepatitis A virus RNA, but not poliovirus RNA, are required for infectivity.

Authors:  S A Harmon; O C Richards; D F Summers; E Ehrenfeld
Journal:  J Virol       Date:  1991-05       Impact factor: 5.103

10.  The tobacco mosaic virus assembly origin RNA. Functional characteristics defined by directed mutagenesis.

Authors:  D R Turner; L E Joyce; P J Butler
Journal:  J Mol Biol       Date:  1988-10-05       Impact factor: 5.469
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  16 in total

1.  Identification of a cis-acting replication element within the poliovirus coding region.

Authors:  I Goodfellow; Y Chaudhry; A Richardson; J Meredith; J W Almond; W Barclay; D J Evans
Journal:  J Virol       Date:  2000-05       Impact factor: 5.103

2.  Coxsackievirus expression of the murine secretory protein interleukin-4 induces increased synthesis of immunoglobulin G1 in mice.

Authors:  N M Chapman; K S Kim; S Tracy; J Jackson; K Höfling; J S Leser; J Malone; P Kolbeck
Journal:  J Virol       Date:  2000-09       Impact factor: 5.103

3.  Density-dependent selection in vesicular stomatitis virus.

Authors:  Isabel S Novella; Daniel D Reissig; Claus O Wilke
Journal:  J Virol       Date:  2004-06       Impact factor: 5.103

4.  Efficient replication of recombinant Enterovirus B types, carrying different P1 genes in the coxsackievirus B5 replicative backbone.

Authors:  Nina Jonsson; Anna Sävneby; Maria Gullberg; Kim Evertsson; Karin Klingel; A Michael Lindberg
Journal:  Virus Genes       Date:  2015-02-08       Impact factor: 2.332

5.  A surrogate assay for measuring Coxsackievirus A6 neutralizing antibodies.

Authors:  Yao Su; Pan Chen; Fan Gao; Lianlian Bian; Shiyang Sun; Fangyu Dong; Yalin Hu; Qunying Mao; Wei Jiang; Xing Wu; Zhenglun Liang
Journal:  Hum Vaccin Immunother       Date:  2018-08-17       Impact factor: 3.452

6.  Limits of variation, specific infectivity, and genome packaging of massively recoded poliovirus genomes.

Authors:  Yutong Song; Oleksandr Gorbatsevych; Ying Liu; JoAnn Mugavero; Sam H Shen; Charles B Ward; Emmanuel Asare; Ping Jiang; Aniko V Paul; Steffen Mueller; Eckard Wimmer
Journal:  Proc Natl Acad Sci U S A       Date:  2017-09-25       Impact factor: 11.205

7.  5'-Terminal deletions occur in coxsackievirus B3 during replication in murine hearts and cardiac myocyte cultures and correlate with encapsidation of negative-strand viral RNA.

Authors:  K-S Kim; S Tracy; W Tapprich; J Bailey; C-K Lee; K Kim; W H Barry; N M Chapman
Journal:  J Virol       Date:  2005-06       Impact factor: 5.103

8.  Formation of the poliovirus replication complex requires coupled viral translation, vesicle production, and viral RNA synthesis.

Authors:  D Egger; N Teterina; E Ehrenfeld; K Bienz
Journal:  J Virol       Date:  2000-07       Impact factor: 5.103

9.  MicroRNA antagonism of the picornaviral life cycle: alternative mechanisms of interference.

Authors:  Elizabeth J Kelly; Elizabeth M Hadac; Bryan R Cullen; Stephen J Russell
Journal:  PLoS Pathog       Date:  2010-03-19       Impact factor: 6.823

10.  Direct interaction between two viral proteins, the nonstructural protein 2C and the capsid protein VP3, is required for enterovirus morphogenesis.

Authors:  Ying Liu; Chunling Wang; Steffen Mueller; Aniko V Paul; Eckard Wimmer; Ping Jiang
Journal:  PLoS Pathog       Date:  2010-08-26       Impact factor: 6.823
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