Literature DB >> 9724411

Transformation of the phytopathogen Mycosphaerella graminicola to carbendazim and hygromycin B resistance.

A C Payne1, M C Grosjean-Cournoyer, D W Hollomon.   

Abstract

The fungal wheat pathogen Mycosphaerella graminicola was transformed to carbendazim and hygromycin B resistance. A beta-tubulin gene from a M. graminicola strain resistant to the fungicide carbendazim was cloned and used to transform a sensitive strain to carbendazim resistance. Hygromycin B-resistant transformants (up to 8 per microgram of transforming DNA) arose at a higher rate than beta-tubulin transformants (up to 0.6 per microgram of DNA). Transformants were able to infect wheat and were morphologically similar to recipient strains.

Entities:  

Mesh：

Substances：

Year:  1998        PMID： 9724411     DOI： 10.1007/s002940050372

Source DB:  PubMed          Journal:  Curr Genet        ISSN： 0172-8083            Impact factor:   3.886


  6 in total

1.  Gene discovery and gene function assignment in filamentous fungi.

Authors:  L Hamer; K Adachi; M V Montenegro-Chamorro; M M Tanzer; S K Mahanty; C Lo; R W Tarpey; A R Skalchunes; R W Heiniger; S A Frank; B A Darveaux; D J Lampe; T M Slater; L Ramamurthy; T M DeZwaan; G H Nelson; J R Shuster; J Woessner; J E Hamer
Journal:  Proc Natl Acad Sci U S A       Date:  2001-04-10       Impact factor: 11.205

Review 2.  A silver bullet in a golden age of functional genomics: the impact of Agrobacterium-mediated transformation of fungi.

Authors:  Alexander Idnurm; Andy M Bailey; Timothy C Cairns; Candace E Elliott; Gary D Foster; Giuseppe Ianiri; Junhyun Jeon
Journal:  Fungal Biol Biotechnol       Date:  2017-09-26

3.  ABC transporters of the wheat pathogen Mycosphaerella graminicola function as protectants against biotic and xenobiotic toxic compounds.

Authors:  L-H Zwiers; I Stergiopoulos; M M C Gielkens; S D Goodall; M A De Waard
Journal:  Mol Genet Genomics       Date:  2003-05-24       Impact factor: 3.291

4.  The bacterial transposon Tn7 causes premature polyadenylation of mRNA in eukaryotic organisms: TAGKO mutagenesis in filamentous fungi.

Authors:  Clive Lo; Kiichi Adachi; Jeffrey R Shuster; John E Hamer; Lisbeth Hamer
Journal:  Nucleic Acids Res       Date:  2003-08-15       Impact factor: 16.971

5.  Construction and high-throughput phenotypic screening ofZymoseptoria tritici over-expression strains.

Authors:  T C Cairns; Y S Sidhu; Y K Chaudhari; N J Talbot; D J Studholme; K Haynes
Journal:  Fungal Genet Biol       Date:  2015-06       Impact factor: 3.495

6.  Exploitation of sulfonylurea resistance marker and non-homologous end joining mutants for functional analysis in Zymoseptoria tritici.

Authors:  Y S Sidhu; T C Cairns; Y K Chaudhari; J Usher; N J Talbot; D J Studholme; M Csukai; K Haynes
Journal:  Fungal Genet Biol       Date:  2015-06       Impact factor: 3.495
  6 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.