Literature DB >> 972072

The quantification of ormazans in tissue sections by microdensitometry. III. The effect of objective power and scanning spot size.

F P Altman.   

Abstract

In this article, it is reported that the formazans derived from neotetrazolium chloride (NT) and 2-(2-benzothiazolyl)-3-(4-phthalhydrazidyl)-5-styryl-tetrazolium chloride (BPST) can be measured by microdensitometry of tissue sections with a wide range of scanning spot sizes, without any significant effect on the recorded extinctions. The spot sizes tested ranged from 0.20 to 16 mum. Thus when large fields have to be measured, it is possible to use low-power objectives and still obtain valid results.

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Year:  1976        PMID： 972072     DOI： 10.1007/BF01003840

Source DB:  PubMed          Journal:  Histochem J        ISSN： 0018-2214


  4 in total

1.  Nonosmiophilic tetrazolium salts that yield osmiophilic, lipophobic formazans for ultrastructural localization of dehydrogenase activity.

Authors:  M Kalina; R E Plapinger; Y Hoshino; A M Seligman
Journal:  J Histochem Cytochem       Date:  1972-09       Impact factor: 2.479

2.  Studies on the reduction of tetrazolium salts. II. The measurement of the half reduced and fully reduced formazans of neotetrazolium chloride in tissue sections.

Authors:  R G Butcher; F P Altman
Journal:  Histochemie       Date:  1973-12-31

3.  The quantification of formazans in tissue sections by microdensitometry. II. The use of BPST, a new tetrazolium salt.

Authors:  F P Altman
Journal:  Histochem J       Date:  1976-09

4.  The quantification of formazans in tissue sections by microdensitometry. I. The use of neotetrazolium chloride.

Authors:  F P Altman
Journal:  Histochem J       Date:  1976-07
  4 in total
  10 in total

1.  The measurement of histochemically-stained NADP-dependent isocitrate dehydrogenase in the sebaceous glands of hairless hamster.

Authors:  W Picton; C Clark
Journal:  Histochem J       Date:  1978-03

2.  Quantitative succinic dehydrogenases histochemistry. A comparison of different tetrazolium salts.

Authors:  H J Lippold
Journal:  Histochemistry       Date:  1982

3.  The radial distribution of succinate dehydrogenase activity in porcine muscle fibres.

Authors:  H J Swatland
Journal:  Histochem J       Date:  1984-03

4.  The determination by microdensitometry of the initial maximum velocity rate of rat ovarian 3 beta hydroxysteroid dehydrogenase activity.

Authors:  W R Robertson; R J Earnshaw; A Lambert
Journal:  Histochemistry       Date:  1982

5.  A quantitative histochemical technique for the characterisation of alpha-glucosidases in the brush-border membrane of rat jejunum.

Authors:  S Gutschmidt; W Kaul; E O Riecken
Journal:  Histochemistry       Date:  1979-09

6.  Reaction rate studies of glucose-6-phosphate dehydrogenase activity in sections of rat liver using four tetrazolium salts.

Authors:  R G Butcher; C J Van Noorden
Journal:  Histochem J       Date:  1985-09

7.  Tetrazolium salts: a consumer's guide.

Authors:  F P Altman
Journal:  Histochem J       Date:  1976-09

8.  Enzyme histochemical reactions in unfixed and undecalcified cryostat sections of mouse knee joints with special reference to arthritic lesions.

Authors:  C J Van Noorden; I M Vogels
Journal:  Histochemistry       Date:  1986

9.  Quantitative histochemical determination of succinic dehydrogenase activity in skeletal muscle fibres.

Authors:  C E Blanco; G C Sieck; V R Edgerton
Journal:  Histochem J       Date:  1988-04

10.  Kinetic behaviour of succinate dehydrogenase of three fibre types in skeletal muscle. I. Effects of temperature and a competitive inhibitor.

Authors:  Y Nakae; M Shono
Journal:  Histochem J       Date:  1984-11
  10 in total

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