Literature DB >> 9719542

Immunoblotting of multiple antigenic peptides.

B T Kurien1, K Jackson, R H Scofield.   

Abstract

Multiple antigenic peptides (MAPs) can be efficiently separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a nitrocellulose membrane for subsequent use in immunoblot (IgG and IgM). MAPs can be stained by Coomassie and silver on SDS-PAGE as well as by Fast Green on an immunoblot. Affinity immunoblotting for analysis of antibody clonotype distribution has also been carried out using MAPs. High performance liquid chromatography purification of the MAPs is mainly responsible for their migration as sharp bands in SDS-PAGE and immunoblotting.

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Year:  1998        PMID： 9719542     DOI： 10.1002/elps.1150191023

Source DB:  PubMed          Journal:  Electrophoresis        ISSN： 0173-0835            Impact factor:   3.535


  8 in total

1.  Induction of anti-Ro60/anti-La by immunisation with spectrin and induction of anti-spectrin by immunisation with Ro60 and 4-hydroxy-2-nonenal-modified Ro60 immunisation.

Authors:  Biji T Kurien; Yaser Dorri; Michael Bachmann; R Hal Scofield
Journal:  Clin Exp Rheumatol       Date:  2012-12-17       Impact factor: 4.473

2.  Passive transfer of antibodies to the linear epitope 60 kD Ro 273-289 induces features of Sjögren's syndrome in naive mice.

Authors:  J S Maier-Moore; B T Kurien; A D'Souza; L Bockus; S Asfa; Y Dorri; S Hubbell; O Yeliosof; D Obeso; T R Schoeb; R Jonsson; R H Scofield
Journal:  Clin Exp Immunol       Date:  2015-04       Impact factor: 4.330

3.  Immunization with 60 kD Ro peptide produces different stages of preclinical autoimmunity in a Sjögren's syndrome model among multiple strains of inbred mice.

Authors:  B T Kurien; A Dsouza; A Igoe; Y J Lee; J S Maier-Moore; T Gordon; M Jackson; R H Scofield
Journal:  Clin Exp Immunol       Date:  2013-07       Impact factor: 4.330

4.  Heat-solubilized curry spice curcumin inhibits antibody-antigen interaction in in vitro studies: a possible therapy to alleviate autoimmune disorders.

Authors:  Biji T Kurien; Anil D'Souza; R Hal Scofield
Journal:  Mol Nutr Food Res       Date:  2010-08       Impact factor: 5.914

5.  Oral administration of heat-solubilized curcumin for potentially increasing curcumin bioavailability in experimental animals.

Authors:  Biji T Kurien; R Hal Scofield
Journal:  Int J Cancer       Date:  2009-10-15       Impact factor: 7.396

6.  Inhibition of p300 and nuclear factor-kappaB by curcumin and its role in diabetic nephropathy.

Authors:  Biji T Kurien
Journal:  Nutrition       Date:  2009-09       Impact factor: 4.008

7.  Bubbling hookah smoke through heat-solubilized curcumin/turmeric and incorporation of the curry spice as an additive or filter in cigarettes to minimize tobacco smoke-related toxicants.

Authors:  Biji T Kurien; R Hal Scofield
Journal:  Med Hypotheses       Date:  2009-05-10       Impact factor: 1.538

8.  Reactivity to the p305 Epitope of the α1G T-Type Calcium Channel and Autoimmune-Associated Congenital Heart Block.

Authors:  Androo J Markham; Sara E Rasmussen; Jane E Salmon; Wilnelly Martinez-Ortiz; Timothy J Cardozo; Robert M Clancy; Jill P Buyon
Journal:  J Am Heart Assoc       Date:  2015-05-20       Impact factor: 5.501
  8 in total

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