Literature DB >> 9718584

The isolation of genomic DNA from blackcurrant (Ribes nigrum L.).

M Woodhead1, H V Davies, R M Brennan, M A Taylor.   

Abstract

A method is described for isolating DNA of high molecular mass (M(r)) from blackcurrant and other softfruit species. Following a hexacethylytimethyl ammonium bromide (CTAB)-based extraction procedure, samples are treated with a glycosidic hydrolase mixture and RNase, and then purified. The suitability of this DNA for Southern analysis and genomic-library construction is demonstrated.

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Year:  1998        PMID： 9718584     DOI： 10.1007/bf02915797

Source DB:  PubMed          Journal:  Mol Biotechnol        ISSN： 1073-6085            Impact factor:   2.695


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