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Literature DB >> 9714601

Digital imaging microscopy of living cells.

Abstract

Fluorescence microscopy has undergone a resurgence in interest following the discovery of green-fluorescent protein (GFP) and its increasing use in live-cell imaging. This article describes an enhanced form of epifluorescence microscopy, digital imaging microscopy, that can be used to produce high-resolution three-dimensional images of samples labelled with GFP, or other fluorochromes, using simple instrumentation and image-restoration software.

Mesh：

Year: 1998 PMID： 9714601 DOI： 10.1016/s0962-8924(98)01301-4

Source DB: PubMed Journal: Trends Cell Biol ISSN： 0962-8924 Impact factor: 20.808

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Cited

14 in total

Review 1. Fluorescent RNA cytochemistry: tracking gene transcripts in living cells.

Authors: T Pederson
Journal: Nucleic Acids Res Date: 2001-03-01 Impact factor: 16.971

2. Dynamics of the mitochondrial reticulum in live cells using Fourier imaging correlation spectroscopy and digital video microscopy.

Authors: D Margineantu; R A Capaldi; A H Marcus
Journal: Biophys J Date: 2000-10 Impact factor: 4.033

10. Phosphatidylinositol-4,5-bisphosphate-rich plasma membrane patches organize active zones of endocytosis and ruffling in cultured adipocytes.

Authors: Shaohui Huang; Larry Lifshitz; Varsha Patki-Kamath; Richard Tuft; Kevin Fogarty; Michael P Czech
Journal: Mol Cell Biol Date: 2004-10 Impact factor: 4.272

Digital imaging microscopy of living cells.

Review 1. Fluorescent RNA cytochemistry: tracking gene transcripts in living cells.

2. Dynamics of the mitochondrial reticulum in live cells using Fourier imaging correlation spectroscopy and digital video microscopy.

3. Diffusion-based transport of nascent ribosomes in the nucleus.

4. Unconventional myosin Myo1c promotes membrane fusion in a regulated exocytic pathway.

5. Differential recruitment of PKC isoforms in HeLa cells during redox stress.

6. Imaging system for visualization and numerical analysis of cancer at stomach and skin tissues.

7. Astrocytic clasmatodendrosis in hippocampal organ culture.

8. Influence of FGF2 and PEG hydrogel matrix properties on hMSC viability and spreading.

9. Insulin action on GLUT4 traffic visualized in single 3T3-l1 adipocytes by using ultra-fast microscopy.

10. Phosphatidylinositol-4,5-bisphosphate-rich plasma membrane patches organize active zones of endocytosis and ruffling in cultured adipocytes.