BACKGROUND: This study characterized the phenotypic subsets of isolated gastric lymphocytes and the cellular immune response in cultured gastric biopsy specimens. METHODS: Endoscopy specimens from 40 Helicobacter pylori-positive and 40 H. pylori-negative patients were studied. a) Isolated gastric lymphocytes were analysed for CD4+, CD8+ T-lymphocyte subsets, activated T cells, and natural killer cells on a fluorescence-activated cell sorter, using monoclonal antibodies. b) The supernatant of cultured gastric biopsy specimens were assayed for interleukin (IL)-2, IL-4, and IL-6 levels. RESULTS: In H. pylori-positive patients there was (a) a decrease in CD4+/CD8+ T cells, no change in activated T cells, and an increase in natural killer cells, and (b) no change in IL-2 levels and a significant increase in IL-4 and IL-6 levels. CONCLUSIONS: There is an increase in CD8+ lymphocytes and natural killer cells, and the observed increase in IL-4 and IL-6 might be important in H. pylori-associated gastritis.
BACKGROUND: This study characterized the phenotypic subsets of isolated gastric lymphocytes and the cellular immune response in cultured gastric biopsy specimens. METHODS: Endoscopy specimens from 40 Helicobacter pylori-positive and 40 H. pylori-negative patients were studied. a) Isolated gastric lymphocytes were analysed for CD4+, CD8+ T-lymphocyte subsets, activated T cells, and natural killer cells on a fluorescence-activated cell sorter, using monoclonal antibodies. b) The supernatant of cultured gastric biopsy specimens were assayed for interleukin (IL)-2, IL-4, and IL-6 levels. RESULTS: In H. pylori-positive patients there was (a) a decrease in CD4+/CD8+ T cells, no change in activated T cells, and an increase in natural killer cells, and (b) no change in IL-2 levels and a significant increase in IL-4 and IL-6 levels. CONCLUSIONS: There is an increase in CD8+ lymphocytes and natural killer cells, and the observed increase in IL-4 and IL-6 might be important in H. pylori-associated gastritis.
Authors: Anna Lundgren; Christina Trollmo; Anders Edebo; Ann-Mari Svennerholm; B Samuel Lundin Journal: Infect Immun Date: 2005-09 Impact factor: 3.441
Authors: M I Esteves; M D Schrenzel; R P Marini; N S Taylor; S Xu; S Hagen; Y Feng; Z Shen; J G Fox Journal: Am J Pathol Date: 2000-02 Impact factor: 4.307
Authors: G Rossi; D Fortuna; L Pancotto; G Renzoni; E Taccini; P Ghiara; R Rappuoli; G Del Giudice Journal: Infect Immun Date: 2000-08 Impact factor: 3.441
Authors: Mai Ping Tan; John Pedersen; Yifan Zhan; Andrew M Lew; Martin J Pearse; Odilia L C Wijburg; Richard A Strugnell Journal: Infect Immun Date: 2007-11-19 Impact factor: 3.441