Literature DB >> 970934

Methionine overproduction by Saccharomycopsis lipolytica.

E Morzycka, D Sawnor-Korszyńska, A Paszewski, J Grabski, K Raczyńska-Bojanowska.   

Abstract

Six ethionine-resistant (Etr) regulatory mutants of Saccharomycopsis lipolytica Sl/1 overproducing methionine have been isolated. Five of them are also resistant to seleno-methionine. The activity of homocysteine synthase (O-acetyl-L-hormoserine-acetate lyase, adding hydrogen sulfide) is derepressed in these mutants and is not susceptible to the methionine-mediated repression. The pool of free methionine in Etr mutants is enhanced 1.5 to 18 times, and incorporation of 35S into methionine is 1.5 to 50 times higher than that in the wild strain. Neither accumulation of endogenous free methionine in Etr mutants nor the uptake of exogenous methionine is accompanied by an increase in the S-adenosylmethionine pool. This implies compartmentation of methionine metabolism in S. lipolytica.

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Year:  1976        PMID： 970934      PMCID： PMC170017          DOI： 10.1128/aem.32.1.125-130.1976

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  11 in total

1.  [Physiological variations of yeast during growth on lactic acid as sole source of carbon].

Authors:  P GALZY; P P SLONIMSKI
Journal:  C R Hebd Seances Acad Sci       Date:  1957-12-16

2.  A spectrophotometric method for the direct determination of cysteine in the presence of other naturally occurring amino acids.

Authors:  M K Gaitonde
Journal:  Biochem J       Date:  1967-08       Impact factor: 3.857

3.  Methionine biosynthesis in Saccharomyces cerevisiae: mutations at the regulatory locus ETH2. II. Physiological and biochemical data.

Authors:  M Masselot; H de Robichon-Szulmajster
Journal:  Mol Gen Genet       Date:  1974-04-03

4.  An Aspergillus nidulans mutant lacking cystathionine -synthase: identity of L-serine sulfhydrylase with cystathionine -synthase and its distinctness from O-acetyl-L-serine sulfhydrylase.

Authors:  N Pieniazek; P P Stepień; A Paszewski
Journal:  Biochim Biophys Acta       Date:  1973-01-24

5.  Regulation of S-amino acids biosynthesis in Aspergillus nidulans. Role of cysteine and-or homocysteine as regulatory effectors.

Authors:  A Paszewski; J Grabski
Journal:  Mol Gen Genet       Date:  1974

6.  The methionine pool and lincomycin biosynthesis in Streptomyces lincolnensis.

Authors:  J Michalik; K Raczyńska-Bojanowska
Journal:  Bull Acad Pol Sci Biol       Date:  1974

7.  Methods for the analysis and preparation of adenosylmethionine and adenosylhomocysteine.

Authors:  S K Shapiro; D J Ehninger
Journal:  Anal Biochem       Date:  1966-05       Impact factor: 3.365

8.  Four new combinations in yeasts.

Authors:  D Yarrow
Journal:  Antonie Van Leeuwenhoek       Date:  1972       Impact factor: 2.271

9.  Genetic analysis of mating type and alkane utilization in Saccharomycopsis lipolytica.

Authors:  J Bassel; R Mortimer
Journal:  J Bacteriol       Date:  1973-05       Impact factor: 3.490

10.  The enzymic synthesis of L-cysteine in Escherichia coli and Salmonella typhimurium.

Authors:  N M Kredich; G M Tomkins
Journal:  J Biol Chem       Date:  1966-11-10       Impact factor: 5.157
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  4 in total

1.  Regulation of s-amino acids biosynthesis in Saccharomycopsis lipolytica.

Authors:  E Morzycka; A Paszewski
Journal:  Mol Gen Genet       Date:  1979-07-02

2.  Methionine-defective mutants in Candida utilis.

Authors:  J M Delgado; A Alonso; X Alvarez; L S Herrera
Journal:  Folia Microbiol (Praha)       Date:  1981       Impact factor: 2.099

3.  Genetic and biochemical study of threonine-overproducing mutants of Saccharomyces cerevisiae.

Authors:  M A Delgado; J Guerrero; J Conde
Journal:  Mol Cell Biol       Date:  1982-07       Impact factor: 4.272

4.  Production of 10-methyl branched fatty acids in yeast.

Authors:  Hannah G Blitzblau; Andrew L Consiglio; Paulo Teixeira; Donald V Crabtree; Shuyan Chen; Oliver Konzock; Gamuchirai Chifamba; Austin Su; Annapurna Kamineni; Kyle MacEwen; Maureen Hamilton; Vasiliki Tsakraklides; Jens Nielsen; Verena Siewers; A Joe Shaw
Journal:  Biotechnol Biofuels       Date:  2021-01-07       Impact factor: 6.040
  4 in total

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