BACKGROUND: Telomerase activity has been shown to be increased in numerous tumors and cell lines, although to the authors' knowledge there has been no previous assessment of telomerase activity in renal cell carcinoma (RCC). To examine whether telomerase activity could be used as a biochemical parameter for predicting the behavior of RCC, telomerase activity was quantified in RCC samples and correlated with clinicopathologic findings. METHODS: Telomerase activity was quantified in 50 RCC samples and 21 samples of normal renal tissue using fluorescence-based telomeric repeat amplification. RESULTS: Telomerase activity was detected in 28 of 50 RCC samples (mean +/- standard deviation, 35.03 +/- 18.47 U/microg protein), whereas there was no telomerase activity in 21 normal renal tissue specimens. Telomerase activity in tumors classified as pT4 was significantly higher than in tumors classified as pT1 to pT3. Telomerase activity also correlated with patient age. CONCLUSIONS: Telomerase activity was increased in RCC samples compared with normal renal tissues. Although significant associations were observed between telomerase activity and clinicopathologic parameters, further study is required to determine whether telomerase activity affects the biologic and clinical behavior of RCC.
BACKGROUND: Telomerase activity has been shown to be increased in numerous tumors and cell lines, although to the authors' knowledge there has been no previous assessment of telomerase activity in renal cell carcinoma (RCC). To examine whether telomerase activity could be used as a biochemical parameter for predicting the behavior of RCC, telomerase activity was quantified in RCC samples and correlated with clinicopathologic findings. METHODS: Telomerase activity was quantified in 50 RCC samples and 21 samples of normal renal tissue using fluorescence-based telomeric repeat amplification. RESULTS: Telomerase activity was detected in 28 of 50 RCC samples (mean +/- standard deviation, 35.03 +/- 18.47 U/microg protein), whereas there was no telomerase activity in 21 normal renal tissue specimens. Telomerase activity in tumors classified as pT4 was significantly higher than in tumors classified as pT1 to pT3. Telomerase activity also correlated with patient age. CONCLUSIONS: Telomerase activity was increased in RCC samples compared with normal renal tissues. Although significant associations were observed between telomerase activity and clinicopathologic parameters, further study is required to determine whether telomerase activity affects the biologic and clinical behavior of RCC.
Authors: Mitchell J Machiela; Jonathan N Hofmann; Robert Carreras-Torres; Kevin M Brown; Mattias Johansson; Zhaoming Wang; Matthieu Foll; Peng Li; Nathaniel Rothman; Sharon A Savage; Valerie Gaborieau; James D McKay; Yuanqing Ye; Marc Henrion; Fiona Bruinsma; Susan Jordan; Gianluca Severi; Kristian Hveem; Lars J Vatten; Tony Fletcher; Kvetoslava Koppova; Susanna C Larsson; Alicja Wolk; Rosamonde E Banks; Peter J Selby; Douglas F Easton; Paul Pharoah; Gabriella Andreotti; Laura E Beane Freeman; Stella Koutros; Demetrius Albanes; Satu Mannisto; Stephanie Weinstein; Peter E Clark; Todd E Edwards; Loren Lipworth; Susan M Gapstur; Victoria L Stevens; Hallie Carol; Matthew L Freedman; Mark M Pomerantz; Eunyoung Cho; Peter Kraft; Mark A Preston; Kathryn M Wilson; J Michael Gaziano; Howard S Sesso; Amanda Black; Neal D Freedman; Wen-Yi Huang; John G Anema; Richard J Kahnoski; Brian R Lane; Sabrina L Noyes; David Petillo; Leandro M Colli; Joshua N Sampson; Celine Besse; Helene Blanche; Anne Boland; Laurie Burdette; Egor Prokhortchouk; Konstantin G Skryabin; Meredith Yeager; Mirjana Mijuskovic; Miodrag Ognjanovic; Lenka Foretova; Ivana Holcatova; Vladimir Janout; Dana Mates; Anush Mukeriya; Stefan Rascu; David Zaridze; Vladimir Bencko; Cezary Cybulski; Eleonora Fabianova; Viorel Jinga; Jolanta Lissowska; Jan Lubinski; Marie Navratilova; Peter Rudnai; Neonila Szeszenia-Dabrowska; Simone Benhamou; Geraldine Cancel-Tassin; Olivier Cussenot; H Bas Bueno-de-Mesquita; Federico Canzian; Eric J Duell; Börje Ljungberg; Raviprakash T Sitaram; Ulrike Peters; Emily White; Garnet L Anderson; Lisa Johnson; Juhua Luo; Julie Buring; I-Min Lee; Wong-Ho Chow; Lee E Moore; Christopher Wood; Timothy Eisen; James Larkin; Toni K Choueiri; G Mark Lathrop; Bin Tean Teh; Jean-Francois Deleuze; Xifeng Wu; Richard S Houlston; Paul Brennan; Stephen J Chanock; Ghislaine Scelo; Mark P Purdue Journal: Eur Urol Date: 2017-08-07 Impact factor: 20.096